Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of white blood cells from healthy human males and females to explore the feasibility of gene expression profiles as a general diagnostic tool

ABSTRACT: The aim of the project was to explore the feasibility of gene expression profiles as a general diagnostic tool. Therefore the most frequently used and easy to use white blood cells have been profiled. We wanted to examine, whether phenotypes created by organs different from blood cells have enough influence on these cells to allow the diagnosis of their presence. As a proof of principle we tried to identify surrogate markers for human sex (excluding markers on sex chromosomes). Experiment Overall Design: 14 HG-U95A and 9 HG-U95Av2 arrays have been hybridized with material from 5 male and 5 female probands with no known diseases; different number of replicates in the range 1 to 4 resulting into 12 experiments in the male group and 11 experiments in the female group.

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Eisenacher

PROVIDER: E-GEOD-3115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Plasmodium falciparum gametocyte stages represent a small fraction of the entire parasite biomass that is present during human malaria infection, yet they alone lead to the transmission of this devastating disease. One of the critical gaps in malaria transmission biology and surveillance is our lack of knowledge about gametocyte biology, especially sexual dimorphic development that may influence transmission from the human to the mosquito. Ratios of male and female gametocytes in the peripheral blood can vary significantly; influenced in part by asexual blood stage and gametocyte density as well as vertebrate and invertebrate host factors. Moreover, the role of sex ratios on gametocyte transmission potential to mosquitoes is unknown and dissecting this process has been hampered by the lack of sex-specific protein markers for the circulating, mature stage V gametocytes. The current evidence suggests a high degree of conservation in gametocyte gene complement across Plasmodium, and therefore presumably for sex-specific genes as well. Therefore, to better our understanding of gametocyte development and subsequent infectiousness to mosquitoes, we undertook a two pronged approach. First, we acquired the mixed, male and female stage V gametocyte proteomes of the NF54 isolate and mature stage V female proteome from Dd2, a strain that is defective in producing mature males. Second, we then undertook a Systematic Subtractive Bioinformatic analysis (filtering) approach to identify sex-specific P. falciparum NF54 protein markers based on a comparison with the Dd2 strain and syntenic male and female proteins from the reanalyzed and updated P. berghei (related rodent malaria parasite) gametocyte proteomes. This has produced a short list of putative 174 male- and 258 female-specific P. falciparum stage V proteins. Furthermore, we generated antibodies against three putative female-specific gametocyte stage V proteins in P. falciparum and confirmed sex-specificity for two proteins and also the loss sex-partitioning for a putative female-specific protein in rodent malaria parasites.

Dataset Information

Transcription profiling of white blood cells from healthy human males and females to explore the feasibility of gene expression profiles as a general diagnostic tool

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