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MRNA-Seq of head tissue from Doa and fne mutants, Drosophila melanogaster.

ABSTRACT: Transcriptional profiling of head tissue of Drosophila Darkener of apricot (Doa) and found in neurons (fne) mutants was performed by RNA-SEQ in females and males. The transcriptional and post-transcriptional targets of these genes, which are involved in regulation by several processes including splicing and mRNA stability, are examined and compared to wild-type. Examination of mRNA profiles in heads of wild-type male and female adult flies are compared to mutant

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Brian Oliver

PROVIDER: E-GEOD-37811 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Design of RNA splicing analysis null models for post hoc filtering of Drosophila head RNA-Seq data with the splicing analysis kit (Spanki).

Sturgill David D Malone John H JH Sun Xia X Smith Harold E HE Rabinow Leonard L Samson Marie-Laure ML Oliver Brian B

BMC bioinformatics 20131109

<h4>Background</h4>The production of multiple transcript isoforms from one gene is a major source of transcriptome complexity. RNA-Seq experiments, in which transcripts are converted to cDNA and sequenced, allow the resolution and quantification of alternative transcript isoforms. However, methods to analyze splicing are underdeveloped and errors resulting in incorrect splicing calls occur in every experiment.<h4>Results</h4>We used RNA-Seq data to develop sequencing and aligner error models. By ...[more]

PMID: 24209455

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