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Gene expression in human umbilical cord endothelial cells following FoxP1 knockdown

ABSTRACT: To search for genes regulated by the transcription factor Forkhead box Protein P1 (FoxP1) in endothelial cells, we transfected human umbilical cord endothelial cells (HUVECs) with a combination of three siRNA oligonucleotides directed against human FoxP1 . Human umbilical vein endothelial cells (HUVECs) were isolated from donated umbilical cords, pooled from two donors and cultivated up to passage 5. For transfection with FoxP1-siRNA cells were cultured to 70% confluence and transfected with a combination of three FoxP1-targeting siRNAs or an irrelevant control oligonucleotide (all from invitrogen) using Lipofectamin RNAiMax (Invitrogen) according to the manufacturers instructions. Total RNA was isolated 48h after transfection. Three individual samples per condition were analyzed.

ORGANISM(S): Homo sapiens

SUBMITTER: Sebastian Grundmann

PROVIDER: E-GEOD-32968 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FoxP1 stimulates angiogenesis by repressing the inhibitory guidance protein semaphorin 5B in endothelial cells.

Grundmann Sebastian S Lindmayer Christian C Hans Felix P FP Hoefer Imo I Helbing Thomas T Pasterkamp Gerard G Bode Christoph C de Kleijn Dominique D Moser Martin M

PloS one 20130902 9

Forkhead box (Fox) transcription factors are important regulators of cardiovascular development and several Fox-proteins have recently been shown to modulate embryonic and post-natal angiogenesis. However, the role of the FoxP subfamily, which is highly expressed in cardiovascular tissue, has not been investigated so far. Here, we show that the transcription factor FoxP1 is the highest expressed FoxP-protein in endothelial cells and that it is upregulated at the site of neovascularization during ...[more]

PMID: 24023716

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