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Unknown,Transcriptomics,Genomics,Proteomics

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Using tiling arrays to precisely detect DNA targets of THO in yeast

ABSTRACT: Crosslinked DNA from wild type cells or from mutant of the THO complex was analyzed by tiling arrays to precisely detect DNA targets of THO in yeast. DNA coming from SDS-washed DCF pellet and the supernatant SN2k of 2 strains were compared in the study, WT (W303) and mft1 delta (DLY224). The DNA of each condition (Pellet or Supernatant) was labeled either with Cy3 or Cy5 fluorescent dyes and competitively hybridized on 244k agilent arrays. For each strain, the experiment was performed on 2 biological replicates, with dye swap.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: sophie lemoine

PROVIDER: E-GEOD-32980 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High-frequency promoter firing links THO complex function to heavy chromatin formation.

Mouaikel John J Causse Sébastien Z SZ Rougemaille Mathieu M Daubenton-Carafa Yves Y Blugeon Corinne C Lemoine Sophie S Devaux Frédéric F Darzacq Xavier X Libri Domenico D

Cell reports 20131107 4

The THO complex is involved in transcription, genome stability, and messenger ribonucleoprotein (mRNP) formation, but its precise molecular function remains enigmatic. Under heat shock conditions, THO mutants accumulate large protein-DNA complexes that alter the chromatin density of target genes (heavy chromatin), defining a specific biochemical facet of THO function and a powerful tool of analysis. Here, we show that heavy chromatin distribution is dictated by gene boundaries and that the gene ...[more]

PMID: 24210826

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