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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional response to stress in serum deprived mouse fibroblasts [RNA-Seq]

ABSTRACT: We have employed gene expression profiling in order to identify targets of transcriptional response to stress in resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin to induce the p38/MAP kinase pathway. Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 1 h (in duplicates). Untreated, serum-starved cells were used as a control. RNA was collected and gene expression profiling using strand-specific RNA-seq was performed.

ORGANISM(S): Mus musculus

SUBMITTER: Anna Sawicka

PROVIDER: E-GEOD-55776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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H3S28 phosphorylation is a hallmark of the transcriptional response to cellular stress.

Sawicka Anna A Hartl Dominik D Goiser Malgorzata M Pusch Oliver O Stocsits Roman R RR Tamir Ido M IM Mechtler Karl K Seiser Christian C

Genome research 20140818 11

The selectivity of transcriptional responses to extracellular cues is reflected by the deposition of stimulus-specific chromatin marks. Although histone H3 phosphorylation is a target of numerous signaling pathways, its role in transcriptional regulation remains poorly understood. Here, for the first time, we report a genome-wide analysis of H3S28 phosphorylation in a mammalian system in the context of stress signaling. We found that this mark targets as many as 50% of all stress-induced genes, ...[more]

PMID: 25135956

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