Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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MRNA-Seq of 2C::tomato+ vs. - ES cells


ABSTRACT: We identified/quantified genes and repeat elements enriched within 2C::tomato+ cells vs. 2C::tomato - cells 2C::tomato + and - cells were collected by FACS for RNA-Seq analysis

ORGANISM(S): Mus musculus

SUBMITTER: Todd Macfarlan 

PROVIDER: E-GEOD-33920 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications


Embryonic stem (ES) cells are derived from blastocyst-stage embryos and are thought to be functionally equivalent to the inner cell mass, which lacks the ability to produce all extraembryonic tissues. Here we identify a rare transient cell population within mouse ES and induced pluripotent stem (iPS) cell cultures that expresses high levels of transcripts found in two-cell (2C) embryos in which the blastomeres are totipotent. We genetically tagged these 2C-like ES cells and show that they lack t  ...[more]

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