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MiRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection

ABSTRACT: miRNAs play an important role in regulating CD8+ T cell response. We used the microarray approach to profile the miRNA signatures of naïve, day 5 effector, day 8 effector, and memory CD8+ T cells. We identified a miRNA signature associated with rapidly proliferating effector CD8+ T cells. CD8+ T cells from P14 TCR transgenic mice were transferred to C57BL6 recipients which were subsequently infected with LCMV Armstrong. Donor P14 CD8+ T cells were sorted on day 5, day 8, or >day 60 post-infection. Naïve P14 CD8+ T cells were sorted directly from naive P14 splenocytes. The total RNA including miRNAs was extracted from sorted samples, labeled, and hybridized to Agilent Mouse miRNA microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Tuoqi Wu

PROVIDER: E-GEOD-34216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Temporal expression of microRNA cluster miR-17-92 regulates effector and memory CD8+ T-cell differentiation.

Wu Tuoqi T Wieland Andreas A Araki Koichi K Davis Carl W CW Ye Lilin L Hale J Scott JS Ahmed Rafi R

Proceedings of the National Academy of Sciences of the United States of America 20120604 25

MicroRNAs are important regulators of various developmental and physiological processes. However, their roles in the CD8(+) T-cell response are not well understood. Using an acute viral infection model, we show that microRNAs of the miR-17-92 cluster are strongly induced after T-cell activation, down-regulated after clonal expansion, and further silenced during memory development. miR-17-92 promotes cell-cycle progression of effector CD8(+) T cells, and its expression is critical to the rapid ex ...[more]

PMID: 22665768

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Temporal expression of miR-17-92a regulates effector and memory CD8+ T cell differentiation

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2011-08-22 | E-GEOD-30962 | biostudies-arrayexpress

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2014-05-07 | E-GEOD-57355 | biostudies-arrayexpress

miRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection

Project description:miRNAs play an important role in regulating CD8+ T cell response. We used the microarray approach to profile the miRNA signatures of naïve, day 5 effector, day 8 effector, and memory CD8+ T cells. We identified a miRNA signature associated with rapidly proliferating effector CD8+ T cells.

2012-06-05 | GSE34216 | GEO

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