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Expression profile of miR-17-92a-MSCV-IRES-Thy1.1 transduced P14 CD8+ T cells

ABSTRACT: During acute viral infections, effector CD8+ T cells differentiate into memory precursors or short-lived terminal effectors. miR-17-92a over-expression skews CD8+ effector cells to the terminal differentiation. We used microarray to identify the genes that are differentially expressed caused by miR-17-92a over-expression. CD8+ T cells from P14 TCR transgenic mice were infected with miR-17-92a-MSCV-IRES-Thy1.1 vector and transfer to C57BL6 recipients. Chimeras were infected with LCMV Armstrong. Thy1.1+ miR-17-92a-MSCV-IRES-Thy1.1 transduced P14 cells and Thy1.1- non-transduced P14 cells were sorted by FACS. RNA was extracted from samples, labeled, and hybridized to Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Tuoqi Wu 

PROVIDER: E-GEOD-34217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Temporal expression of microRNA cluster miR-17-92 regulates effector and memory CD8+ T-cell differentiation.

Wu Tuoqi T   Wieland Andreas A   Araki Koichi K   Davis Carl W CW   Ye Lilin L   Hale J Scott JS   Ahmed Rafi R  

Proceedings of the National Academy of Sciences of the United States of America 20120604 25

MicroRNAs are important regulators of various developmental and physiological processes. However, their roles in the CD8(+) T-cell response are not well understood. Using an acute viral infection model, we show that microRNAs of the miR-17-92 cluster are strongly induced after T-cell activation, down-regulated after clonal expansion, and further silenced during memory development. miR-17-92 promotes cell-cycle progression of effector CD8(+) T cells, and its expression is critical to the rapid ex  ...[more]

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