Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA expression profile in murine advanced atherosclerotic plaque compared with early atherosclerotic plaque


ABSTRACT: Aberrantly expressed miRNAs contribute to developmental abnormalities and diseases such as cancer, diabetes, and cardiovascular disorders, by hybridizing to specific mRNA targets and repressing their translation into proteins. Although miRNA expression signature is characterized in the process of neointimal thickening during proliferative vascular diseases such as atherosclerosis, so far global miRNA expression profiling in the different stages of atherosclerosis is completely unknown. We explored stage-specific microRNA signatures in the progress of atherosclerosis in hyperlipidemia mouse model, which may help to identify the critical miRNAs contributing atherosclerotic development and stabilization. Female apoe-/- mice (6-8 weeks, Jackson Laboratory) were fed a high fat diet (HFD, 21% crude fat, 0.15% cholesterol and 19.5% casein, Altromin, Germany) for 3 or 10 months (N=3-4). Serial sections (20 µm thick) of aortic roots and carotid arteries from these mice were mounted on membrane mounted metal frame slides (MMI), deparaffinized under RNase-free conditions, and completely dried. Laser capture microdissection (LCM) was performed with a laser microdissection system (MMI cellcut plus, Molecular Machines and Industries, Switzerland) assembled onto an inverted microscope (Olympus IX71). Plaque tissue or morphologically normal vessel wall of at least 40 sections per mouse were collected. RNA was isolated using RecoverAll total nucleic acid isolation kit (Applied Biosystems) according to the manufacturer’s instructions.

ORGANISM(S): Mus musculus

SUBMITTER: Andreas Schober 

PROVIDER: E-GEOD-34647 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The microRNA-342-5p fosters inflammatory macrophage activation through an Akt1- and microRNA-155-dependent pathway during atherosclerosis.

Wei Yuanyuan Y   Nazari-Jahantigh Maliheh M   Chan Lily L   Zhu Mengyu M   Heyll Kathrin K   Corbalán-Campos Judit J   Hartmann Petra P   Thiemann Anna A   Weber Christian C   Schober Andreas A  

Circulation 20130319 15


<h4>Background</h4>Atherosclerosis is a chronic inflammatory vascular disease driven by the subendothelial accumulation of macrophages. The mechanism regulating the inflammatory response in macrophages during atherogenesis remains unclear. Because microRNAs (miRNAs) play a crucial role in cellular signaling by posttranscriptional regulation of gene expression, we studied the miRNA expression profiles during the progression of atherosclerosis.<h4>Methods and results</h4>Using an miRNA real-time p  ...[more]

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