Ontology highlight
ABSTRACT:
ORGANISM(S): synthetic construct
SUBMITTER: Scot Wolfe
PROVIDER: E-GEOD-35806 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Chu Stephanie W SW Noyes Marcus B MB Christensen Ryan G RG Pierce Brian G BG Zhu Lihua J LJ Weng Zhiping Z Stormo Gary D GD Wolfe Scot A SA
Genome research 20120426 10
The recognition potential of most families of DNA-binding domains (DBDs) remains relatively unexplored. Homeodomains (HDs), like many other families of DBDs, display limited diversity in their preferred recognition sequences. To explore the recognition potential of HDs, we utilized a bacterial selection system to isolate HD variants, from a randomized library, that are compatible with each of the 64 possible 3' triplet sites (i.e., TAANNN). The majority of these selections yielded sets of HDs wi ...[more]