Unknown,Transcriptomics,Genomics,Proteomics

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Exploring the DNA-Recognition Potential of Homeodomains


ABSTRACT: We utilized a bacterial selection system to isolate Engrailed variants from a randomized library that are compatible with each of the 64 possible 3’ triplet sites (i.e. TAANNN). The DNA-binding specificity of 151 representative HD variants from these populations was subsequently characterized. Many of these variants contain novel combinations of specificity determinants that are uncommon or absent in extant HDs. These novel determinants, when grafted into different HD backbones, produce a corresponding alteration in their specificity. The identified determinates expand our understanding of HD recognition. Homeodomains where selected against each of the 64 possible 3' triplet sites (ie: TAANNN). The DNA-binding specificity of 151 representative HD variants from these populations was subsequently characterized. 7 novel determinants, when grafted into 3 different HD backbones. 5' speicifity was changed for 3 novel determinants.

ORGANISM(S): synthetic construct

SUBMITTER: Scot Wolfe 

PROVIDER: E-GEOD-35806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exploring the DNA-recognition potential of homeodomains.

Chu Stephanie W SW   Noyes Marcus B MB   Christensen Ryan G RG   Pierce Brian G BG   Zhu Lihua J LJ   Weng Zhiping Z   Stormo Gary D GD   Wolfe Scot A SA  

Genome research 20120426 10


The recognition potential of most families of DNA-binding domains (DBDs) remains relatively unexplored. Homeodomains (HDs), like many other families of DBDs, display limited diversity in their preferred recognition sequences. To explore the recognition potential of HDs, we utilized a bacterial selection system to isolate HD variants, from a randomized library, that are compatible with each of the 64 possible 3' triplet sites (i.e., TAANNN). The majority of these selections yielded sets of HDs wi  ...[more]

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