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Discovery of the genes controlled by the second messenger cyclic di-GMP in Mycobacterium tuberculosis

ABSTRACT: Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many biological processes in bacteria. The genome in Mycobacterium tuberculosis encodes a single copy of the diguanylate cyclase gene (dgc) responsible for c-di-GMP synthesis. To determine the role of c-di-GMP signaling in M. tuberculosis, the mutant strain of Δdgc was generated in the virulent H37Rv strain. We used whole genome microarray expression profiling as a discovery platform to identify the genes controlled by c-di-GMP in M. tuberculosis, providing molecular proof for the phenotypes modulated by the signaling. Wild-type H37Rv and Δdgc cultures were analyzed under aerobic conditions or in an in vitro dormancy model. Bacteria were collected at OD600 =1.3 for the aerobic cultures and upon the beginning of anaerobiosis for the cultures in the dormancy model. One culture for each experiment was assayed except for Δdgc under anaerobiosis (2 independent cultures).

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Yuzhi Hong 

PROVIDER: E-GEOD-37973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cyclic di-GMP mediates Mycobacterium tuberculosis dormancy and pathogenecity.

Hong Yuzhi Y   Zhou Xiaodan X   Fang Haihong H   Yu Dan D   Li Chuanyou C   Sun Baolin B  

Tuberculosis (Edinburgh, Scotland) 20130919 6

Dormancy of Mycobacterium tuberculosis is likely to be a major cause of extended chemotherapeutic regimens and wide prevalence of tuberculosis. The molecular mechanisms underlying M. tuberculosis dormancy are not well understood. In this study, single-copy genes responsible for synthesis (dgc) and degradation (pde) of the ubiquitous bacterial second messenger, cyclic di-GMP (c-di-GMP), were deleted in the virulent M. tuberculosis strain H37Rv to generate dgc(mut) and Δpde, respectively. Under ae  ...[more]

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