Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

36 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression Profiles of HepG2 cells treated with 7M-BM-5M of the genotoxic compound cisplatin

ABSTRACT: The transcriptomic changes induced in the human liver cell line HepG2 by 7M-BM-5M of cisplatin after treatment for 12, 24 and 48h The study investigated differential gene expression in HepG2 cell line mRNA following 12,24 and 48 hours of exposure to 7M-BM-5M of cisplatin and its solvent. Three biological replicates per compound/solvent. In total 18 arrays .

ORGANISM(S): Homo sapiens

SUBMITTER: Linda Rieswijk

PROVIDER: E-GEOD-38122 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Characterisation of cisplatin-induced transcriptomics responses in primary mouse hepatocytes, HepG2 cells and mouse embryonic stem cells shows conservation of regulating transcription factor networks.

Rieswijk Linda L Lizarraga Daneida D Brauers Karen J J KJ Kleinjans Jos C S JC van Delft Joost H M JH

Mutagenesis 20131126 1

The toxic mechanisms of cisplatin have been frequently studied in many species and in vitro cell models. The Netherlands Toxicogenomics Centre focuses on developing in vitro alternatives using genomics technologies for animal-based assays on, e.g. genotoxic hazards. Models such as human hepatocellular carcinoma cell line (HepG2) cells, mouse primary hepatocytes (PMH) and mouse embryonic stem cells (mESC) are used. Our aim was to identify possibly robust conserved mechanisms between these models ...[more]

PMID: 24280081

Similar Datasets

Expression Profiles of PMH treated with 7M-BM-5M of the genotoxic compound cisplatin

Project description:The transcriptomic changes induced in primary mouse hepatocytes (C57BL/6 ) by 7M-BM-5M of cisplatin after treatment for 24 and 48h The study investigated differential gene expression in PMH mRNA following 24 and 48 hours of exposure to 7M-BM-5M of cisplatin and its solvent. Three biological replicates per compound/solvent. In total 12 arrays.

2013-12-05 | E-GEOD-38123 | biostudies-arrayexpress

Expression Profiles of HepG2 cells treated with Cyclosporin A and solvent control

Project description:The transcriptomics changes induced in the human liver cell line HepG2 by Cyclosporin A after treatment for 12h, 24h, 48h and 72h The study investigated differential gene expression in HepG2 cell line mRNA following 12, 24, 48 and 72 hours of exposure to Cyclosporin A and solvent. Three biological replicates per compound/solvent. In total 36 arrays.

2015-01-27 | E-GEOD-45635 | biostudies-arrayexpress

Expression Profiles of HepG2 cells treated with following oxidants: 100M-BM-5M menadione, 200M-BM-5M TBH or 50M-BM-5M H2O2

Project description:The transcriptomic changes induced in the human liver cell line HepG2 by 100M-BM-5M menadione, 200M-BM-5M TBH or 50M-BM-5M H2O2 after treatment for 0.5, 1, 2, 4, 6, 8 and 24h. The study investigated differential gene expression in HepG2 cell line mRNA following 0.5, 1, 2, 4, 6, 8 and 24h hours of exposure to 100M-BM-5M menadione, 200M-BM-5M TBH or 50M-BM-5M H2O2 and medium without compound. Three biological replicates per compound/solvent. In total 126 arrays.

2013-05-31 | E-GEOD-39291 | biostudies-arrayexpress

Expression Profiles of HepG2 cells treated with following compounds: Azathriopine, Furan, Tetradecanoyl phorbol acetate, Tetrachloroethylene, Diazinon and Dmannitol

Project description:The transcriptomic changes induced in the human liver cell line HepG2 by Azathriopine (250M-BM-5M, Sigma-Aldrich), Furan (2mM, Sigma-Aldrich), Tetradecanoyl phorbol acetate (500nM, Sigma-Aldrich), Tetrachloroethylene (2mM, Sigma-Aldrich), Diazinon (250M-BM-5M, Sigma-Aldrich) and Dmannitol (250M-BM-5M, Sigma-Aldrich) during 4, 8, 24, 48 and 72hrs As a solvent control for Azathriopine, Furan, Diazinon and Tetradecanoyl phorbol acetate, DMSO was used (0.5%); As a solvent control for tetrachloroethylene, ethanol was used (0.5%); As a solvent control for D-mannitol, HBSS was used (0.5%) The study investigated differential gene expression in HepG2 cell line mRNA following 4, 8, 24, 48 and 72h hours of exposure to Azathriopine, Furan, Tetradecanoyl phorbol acetate, Tetrachloroethylene, Diazinon, D-mannitol, DMSO solvent control, ethanol solvent control and HBSS solvent control. Three biological replicates per compound/solvent. In total 135 arrays were analyzed in total.

2015-01-13 | E-GEOD-58235 | biostudies-arrayexpress

Expression Profiles of HepG2 cells treated with genotoxic and non-genotoxic agents

Project description:The lack of accurate in vitro assays for predicting in vivo toxicity of chemicals together with new legislations demanding replacement and reduction of animal testing has triggered the development of alternative methods. This study aimed at developing a transcriptomics-based in vitro prediction assay for in vivo genotoxicity. The transcriptomics changes induced in the human liver cell line HepG2 by 34 compounds after treatment for 12h, 24h and 48h were used for the selection of gene-sets that can discriminate between in vivo genotoxins (GTX) and in vivo non-genotoxins (NGTX). By combining publicly available results for these chemicals from standard in vitro genotoxicity studies with transcriptomics, we developed several prediction models. These models were validated by means of an additional set of 28 chemicals. The study investigated differential gene expression in HepG2 cell line mRNA following 12 hours of exposure to 34 different compounds and their solvents; 24 and 48 hours of exposure to 62 different compounds and their solvents. Three biological replicates per compound/solvent. In total 560 arrays .

2012-08-02 | E-GEOD-28878 | biostudies-arrayexpress

Expression Profiles of HepG2 cells treated with 22 compounds and solvent controls

Project description:The transcriptomics changes induced in the human liver cell line HepG2 by 17 hepatotoxic compounds, 5 non-hepatotoxic compounds and solvent controls after treatment for 24h The study investigated differential gene expression in HepG2 cell line mRNA following 24 hours of exposure to 17 hepatotoxic compounds, 5 non-hepatotoxic compounds and solvent controls. Three biological replicates per compound/solvent. In total 105 arrays .

2014-02-14 | E-GEOD-51952 | biostudies-arrayexpress

Expression profiles of HepG2 cells treated with low-, high-dose of acetaminophen and solvent control

Project description:The transcriptomics changes induced in the human liver cell line HepG2 by low and high doses of acetaminophen and solvent controls after treatment for 4 time points (12h, 24h, 48h and 72h) The study investigated differential gene expression in HepG2 cell line mRNA following 12 to 72 hours of exposure to low and high doses of acetaminophen and solvent controls. Three biological replicates per compound/solvent.

2015-12-15 | E-GEOD-53216 | biostudies-arrayexpress

Transcription profiling by array of human HepG2 cells treated with TCDD

Project description:The integration of different M-^SomicsM-^T technologies has already been shown in several in vivo studies to offer a complementary insight into cellular responses of toxic processes. We therefore hypothesize that the combining of transcriptomics and metabonomics data may improve the understanding of molecular mechanisms underlying non-genotoxic carcinogenicity in vitro. To test this hypothesis, the human hepatocarcinoma cell line HepG2 was exposed to the well known environmental pollutant TCDD. <br><br>A custom CDF file is available on the FTP site for this experiment (in file E-MEXP-2817.additional.zip) for use with the normalized data file for this experiment.

2011-07-01 | E-MEXP-2817 | biostudies-arrayexpress

Transcription profiling of human hepatoma cell lines HepG2 and HepaRG using whole genome gene expression analysis before and after exposure to the GTX carcinogens AFB1 and BaP, and the NGTX carcinogens CsA, E2 and TCDD for 12 and 48 h

Project description:Direct comparison of the hepatoma cell lines HepG2 and HepaRG using whole genome gene expression analysis before and after exposure to the GTX carcinogens AFB1 and BaP and the NGTX carcinogens CsA, E2 and TCDD for 12 and 48 h.

2010-04-15 | E-MEXP-2458 | biostudies-arrayexpress

Characterization of cisplatin-induced transcriptomics responses in primary mouse hepatocytes, HepG2 cells and mouse embryonic stem cells shows a strong conservation of involved transcription factors

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-12-05 | E-GEOD-38124 | biostudies-arrayexpress