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Expression Profiles of PMH treated with 7M-BM-5M of the genotoxic compound cisplatin

ABSTRACT: The transcriptomic changes induced in primary mouse hepatocytes (C57BL/6 ) by 7M-BM-5M of cisplatin after treatment for 24 and 48h The study investigated differential gene expression in PMH mRNA following 24 and 48 hours of exposure to 7M-BM-5M of cisplatin and its solvent. Three biological replicates per compound/solvent. In total 12 arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Linda Rieswijk

PROVIDER: E-GEOD-38123 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterisation of cisplatin-induced transcriptomics responses in primary mouse hepatocytes, HepG2 cells and mouse embryonic stem cells shows conservation of regulating transcription factor networks.

Rieswijk Linda L Lizarraga Daneida D Brauers Karen J J KJ Kleinjans Jos C S JC van Delft Joost H M JH

Mutagenesis 20131126 1

The toxic mechanisms of cisplatin have been frequently studied in many species and in vitro cell models. The Netherlands Toxicogenomics Centre focuses on developing in vitro alternatives using genomics technologies for animal-based assays on, e.g. genotoxic hazards. Models such as human hepatocellular carcinoma cell line (HepG2) cells, mouse primary hepatocytes (PMH) and mouse embryonic stem cells (mESC) are used. Our aim was to identify possibly robust conserved mechanisms between these models ...[more]

PMID: 24280081

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