Unknown,Transcriptomics,Genomics,Proteomics

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (ChIP-Seq)


ABSTRACT: Characterization of the activities of the transcription factors that AP3 and PI encode throughout flower development using perturbation and ChIPSeq assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. Examination of genomic regions bound by fully functional AP3-GFP and PI-GFP proteins at approx floral stage 4-5 as compared to a negative control sample

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Samuel Wuest 

PROVIDER: E-GEOD-38358 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA.

Wuest Samuel E SE   O'Maoileidigh Diarmuid S DS   Rae Liina L   Kwasniewska Kamila K   Raganelli Andrea A   Hanczaryk Katarzyna K   Lohan Amanda J AJ   Loftus Brendan B   Graciet Emmanuelle E   Wellmer Frank F  

Proceedings of the National Academy of Sciences of the United States of America 20120730 33


How different organs are formed from small sets of undifferentiated precursor cells is a key question in developmental biology. To understand the molecular mechanisms underlying organ specification in plants, we studied the function of the homeotic selector genes APETALA3 (AP3) and PISTILLATA (PI), which control the formation of petals and stamens during Arabidopsis flower development. To this end, we characterized the activities of the transcription factors that AP3 and PI encode throughout flo  ...[more]

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