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Transcription profiling of E. coli K-12 yliH and yceP biofilm expression relative to wild type

ABSTRACT: Expression in biofilm cells of mutants yceP and yliH relative to biofilm cells of E. coli K-12 wild type strain Experiment Overall Design: All strain cultured in LB glu, biofilm isolated from glass wool after culturing for 24 hrs.

ORGANISM(S): Escherichia coli

SUBMITTER: Thomas Wood

PROVIDER: E-GEOD-3937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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YliH (BssR) and YceP (BssS) regulate Escherichia coli K-12 biofilm formation by influencing cell signaling.

Domka Joanna J Lee Jintae J Wood Thomas K TK

Applied and environmental microbiology 20060401 4

We previously discovered that yliH and yceP are induced in Escherichia coli biofilms (D. Ren, L. A. Bedzyk, S. M. Thomas, R. W. Ye, and T. K. Wood, Appl. Microbiol. Biotechnol. 64:515-524, 2004). Here, it is shown that deletion of yceP (b1060) and yliH (b0836) increases biofilm formation in continuous-flow chambers with minimal glucose medium by increasing biofilm mass (240- to 290-fold), surface coverage (16- to 31-fold), and mean thickness (2,800-fold). To determine the genetic basis of the in ...[more]

PMID: 16597943

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