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Transcription profiling of E. coli K-12 mutant ycfR biofilm vs wild type 15 hr LB glu biofilm cells

ABSTRACT: E. coli K-12 BW25113 mutant strain ycfR expression in biofilm cells relative to E. coli wild-type strain expression in biofilm cells. All samples cultured in LB glu with glass wool Experiment Overall Design: Strains: E. coli K-12 BW25113 wild type and mutant ycfR Experiment Overall Design: Medium: LB glu Experiment Overall Design: Biofilm grown on glass wool Experiment Overall Design: Time: 15 hr Experiment Overall Design: Cell type: biofilm

ORGANISM(S): Escherichia coli

SUBMITTER: Rodolfo Garcia Contreras

PROVIDER: E-GEOD-5904 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity.

Zhang Xue-Song XS García-Contreras Rodolfo R Wood Thomas K TK

Journal of bacteriology 20070209 8

DNA microarrays revealed that expression of ycfR, which encodes a putative outer membrane protein, is significantly induced in Escherichia coli biofilms and is also induced by several stress conditions. We show that deletion of ycfR increased biofilm formation fivefold in the presence of glucose; the glucose effect was corroborated by showing binding of the cyclic AMP receptor protein to the ycfR promoter. It appears that YcfR is a multiple stress resistance protein, since deleting ycfR also ren ...[more]

PMID: 17293424

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