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Transcription profiling of mouse day 5 5cc7 T cell blasts

ABSTRACT: Our aim was to get a general sense of which proteins are expressed in 5cc7 T cell blasts. We use these cells for a number of different experiments in our lab Experiment Overall Design: RNA was isolated from day 5 5cc7 T cell blasts. This RNA was converted to cRNA and used to hybridize 2 affymetrix mouse 430 2.0 arrays in parallel

ORGANISM(S): Mus musculus

SUBMITTER: Morgan Huse

PROVIDER: E-GEOD-4050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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T cells use two directionally distinct pathways for cytokine secretion.

Huse Morgan M Lillemeier Björn F BF Kuhns Michael S MS Chen Daniel S DS Davis Mark M MM

Nature immunology 20060129 3

Activated T helper cells produce many cytokines, some of which are secreted through the immunological synapse toward the antigen-presenting cell. Here we have used immunocytochemistry, live-cell imaging and a surface-mediated secretion assay to show that there are two cytokine export pathways in T helper cells. Some cytokines, including interleukin 2 and interferon-gamma, were secreted into the synapse, whereas others, including tumor necrosis factor and the chemokine CCL3 (MIP-1alpha), were rel ...[more]

PMID: 16444260

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