Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

135 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling in psoriatic lesional and non-lesional skin [Set 2]

ABSTRACT: To explore the psoriasis phenotype and pathways involved in psoriasis, we characterized gene expression in lesional and non-lesional skin from psoriasis patients. From each of the 24 psoriasis patients, we obtained two biopsies, one from a lesion and the other from non-lesional skin in the same general body geography. Total RNA was extracted from 6mm punch biopsies that were split in half. A total of 48 samples were run on Affymetrix HU133 Plus 2.0 microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Jeannette Bigler

PROVIDER: E-GEOD-41662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Cross-study homogeneity of psoriasis gene expression in skin across a large expression range.

Bigler Jeannette J Rand Hugh A HA Kerkof Keith K Timour Martin M Russell Christopher B CB

PloS one 20130104 1

<h4>Background</h4>In psoriasis, only limited overlap between sets of genes identified as differentially expressed (psoriatic lesional vs. psoriatic non-lesional) was found using statistical and fold-change cut-offs. To provide a framework for utilizing prior psoriasis data sets we sought to understand the consistency of those sets.<h4>Methodology/principal findings</h4>Microarray expression profiling and qRT-PCR were used to characterize gene expression in PP and PN skin from psoriasis patients ...[more]

PMID: 23308107

Similar Datasets

Gene expression profiling in psoriatic lesional and non-lesional skin [brodalumab treatment]

Project description:To explore the psoriasis phenotype and pathways involved in psoriasis, we characterized gene expression in lesional and non-lesional skin from psoriasis patients. Furthermore, we explored the effects of various doses of brodalumab on lesional skin over time. From each of the 25 psoriasis patients, we obtained two pre-dose biopsies, one from a lesion and the other from non-lesional skin in the same general body geography, and two post-dose biopsies. Total RNA was extracted from 6mm punch biopsies that were split in half. A total of 99 samples were run on Affymetrix HU133 Plus 2.0 microarrays. There was no paired non-lesional sample for 'T_lesional_pre-dose' (skn55789).

2014-03-31 | E-GEOD-53552 | biostudies-arrayexpress

Gene expression profiling in psoriatic lesional and non-lesional skin [Set 1]

Project description:To explore the psoriasis phenotype, we characterize gene expression in lesional and non-lesional skin from psoriasis patients. We extracted total RNA from 5mm punch biopsies taken from 14 psoriatic patients. From each patient, we obtained two biopsies, one from a lesion and the other from non-lesional skin in the same general body geography. A total of 28 samples were run on Affymetrix HU133 Plus 2.0 microarrays.

2013-01-28 | E-GEOD-34248 | biostudies-arrayexpress

Re-analysis by microarray using cDNA target of samples from psoriasis patients enrolled in an etanercept trial

Project description:cDNA and cRNA hybridization technologies have different, probe-specific sensitivities. We used samples from an etanercept trial (GSE11903) to explore in a real-life setting the uniqueness of each platform. We obtained total RNA for a subset of the samples in GSE11903 from Jim Krueger and generated expression profiles using labeled cDNA target. From 14 patients, available base-line lesional and non-lesional skin biopsy pairs as well as post-dose samples were analyzed. All the samples were run on Affymetrix HU133 Plus 2.0 microarrays.

2013-01-28 | E-GEOD-41663 | biostudies-arrayexpress

Comparison of Gene Expression in Psoriatic Skin from Different Sources

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-01-28 | E-GEOD-41664 | biostudies-arrayexpress

Transcription profiling of human skin biposies (lesional and uninvolved) from psoriasis patients

Project description:Biopsies from uninvolved and from lesional skin of 13 patients with plaque-type psoriasis. Based on paired samples, 179 genes were more than 2-fold differentially expressed in lesional skin. Experiment Overall Design: Comparative RNA expression profiles from uninvolved and lesional skin of 13 patients with mild to severe plaque-type psoriasis.

2008-11-07 | E-GEOD-6710 | biostudies-arrayexpress

Effect of rBTNL2.Fc co-stimulation on murine CD4+ cells

Project description:Naïve T cell activation involves at least two signals from an antigen presenting cell (APC), one through the T cell receptor via interaction with APC peptide-MHC complexes and a second through interaction of CD28 with APC B7 ligands. Following activation, T cells up-regulate a host of other membrane-bound costimulatory molecules which can either promote or inhibit further T cell maturation and proliferation. In some cases, it is necessary to attenuate T cell activation to prevent deleterious inflammation, and inhibitory members of the B7/Butyrophilin family of ligands have evolved to balance the strong stimuli the activating B7 ligands confer. Human genetic association and in vitro studies have implicated one such ligand, BTNL2, in controlling inflammation at mucosal surfaces. Here we show that recombinant mouse BTNL2 modifies B7/CD28 signaling to promote expression of Foxp3, a transcription factor necessary for murine Treg development and function. BTNL2 blocks Akt-mediated inactivation of Foxo1, a transcription factor necessary for Foxp3 expression. Immunophenotyping and gene profiling reveal that BTNL2-induced Treg share many properties with natural Treg, and in vivo they suppress enteritis induced by mouse effector T cells. These findings describe a mechanism by which environmental antigen-driven formation of Treg may be orchestrated by APC expressing specific modulators of costimulatory signals. CD4+CD25- cells isolated from spleen were stimulated on plates coated with anti-mouse CD3 and human IgG (negative control), anti-mouse CD3, rB7-2, and IgG (positive control), and anti-mouse CD3, rB7-2, and rBTNL2 (experimental). Culturing was performed in duplicate for each condition.

2013-01-31 | E-GEOD-42385 | biostudies-arrayexpress

Expression data from human skin biposies (lesional and uninvolved) from psoriatic patients

Project description:Biopsies from uninvolved and from lesional skin of 13 patients with plaque-type psoriasis. Based on paired samples, 179 genes were more than 2-fold differentially expressed in lesional skin. Keywords: Comparison of uninvolved and lesional skin

2007-01-12 | GSE6710 | GEO

Gene expression profiling in psoriatic lesional and non-lesional skin [brodalumab treatment]

2014-03-31 | GSE53552 | GEO

Gene expression profiling in psoriatic lesional and non-lesional skin [Set 2]

Project description:To explore the psoriasis phenotype and pathways involved in psoriasis, we characterized gene expression in lesional and non-lesional skin from psoriasis patients.

2013-01-28 | GSE41662 | GEO

Gene expression profiling in psoriatic lesional and non-lesional skin [Set 1]

Project description:To explore the psoriasis phenotype, we characterize gene expression in lesional and non-lesional skin from psoriasis patients.

2013-01-28 | GSE34248 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data