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Gene expression profiling in psoriatic lesional and non-lesional skin [Set 2]

ABSTRACT: To explore the psoriasis phenotype and pathways involved in psoriasis, we characterized gene expression in lesional and non-lesional skin from psoriasis patients. From each of the 24 psoriasis patients, we obtained two biopsies, one from a lesion and the other from non-lesional skin in the same general body geography. Total RNA was extracted from 6mm punch biopsies that were split in half. A total of 48 samples were run on Affymetrix HU133 Plus 2.0 microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Jeannette Bigler

PROVIDER: E-GEOD-41662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cross-study homogeneity of psoriasis gene expression in skin across a large expression range.

Bigler Jeannette J Rand Hugh A HA Kerkof Keith K Timour Martin M Russell Christopher B CB

PloS one 20130104 1

<h4>Background</h4>In psoriasis, only limited overlap between sets of genes identified as differentially expressed (psoriatic lesional vs. psoriatic non-lesional) was found using statistical and fold-change cut-offs. To provide a framework for utilizing prior psoriasis data sets we sought to understand the consistency of those sets.<h4>Methodology/principal findings</h4>Microarray expression profiling and qRT-PCR were used to characterize gene expression in PP and PN skin from psoriasis patients ...[more]

PMID: 23308107

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