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Re-analysis by microarray using cDNA target of samples from psoriasis patients enrolled in an etanercept trial

ABSTRACT: cDNA and cRNA hybridization technologies have different, probe-specific sensitivities. We used samples from an etanercept trial (GSE11903) to explore in a real-life setting the uniqueness of each platform. We obtained total RNA for a subset of the samples in GSE11903 from Jim Krueger and generated expression profiles using labeled cDNA target. From 14 patients, available base-line lesional and non-lesional skin biopsy pairs as well as post-dose samples were analyzed. All the samples were run on Affymetrix HU133 Plus 2.0 microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Jeannette Bigler

PROVIDER: E-GEOD-41663 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cross-study homogeneity of psoriasis gene expression in skin across a large expression range.

Bigler Jeannette J Rand Hugh A HA Kerkof Keith K Timour Martin M Russell Christopher B CB

PloS one 20130104 1

<h4>Background</h4>In psoriasis, only limited overlap between sets of genes identified as differentially expressed (psoriatic lesional vs. psoriatic non-lesional) was found using statistical and fold-change cut-offs. To provide a framework for utilizing prior psoriasis data sets we sought to understand the consistency of those sets.<h4>Methodology/principal findings</h4>Microarray expression profiling and qRT-PCR were used to characterize gene expression in PP and PN skin from psoriasis patients ...[more]

PMID: 23308107

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