Unknown,Transcriptomics,Genomics,Proteomics

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Expression analysis of the first-generation offspring of irradiated male mice

ABSTRACT: Investigation of whole genome gene expression level changes in kidney, brain, liver and spleen of the offspring of CBA/Ca and BALB/c male mice exposed to 1 Gy of acute X-rays The pattern of gene expression of a number of circadian rhythm genes in the offspring of irradiated males is compromised A twenty-two 12x135K chip study using total RNA extracted from kidney, brain, liver and spleen of control CBA/Ca and BALB/c male mice and the first-generation offspring of male mice exposed to 1 Gy of acute X-rays. The expression of 42,575 genes was measured with two-fold technical redundancy.

ORGANISM(S): Mus musculus

SUBMITTER: Yuri Dubrova

PROVIDER: E-GEOD-42621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Measuring global gene expression using cDNA or oligonucleotide microarrays is an effective approach to understanding the complex mechanisms of the effects of radiation. However, few studies have been carried out that investigate gene expression in vivo after prolonged exposure to low-dose-rate radiation. In this study, C57BL/6J mice were continuously irradiated with ?-rays for 485 days at dose-rates of 0.032 – 13 ?Gy/min. Gene expression profiles in the kidney and testis from irradiated and unirradiated mice were analyzed, and differentially expressed genes were identified. A combination of pathway analysis and hierarchical clustering of differentially expressed genes revealed that expression of genes involved in mitochondrial oxidative phosphorylation was elevated in the kidney after irradiation at the dose-rates of 0.65 ?Gy/min and 13 ?Gy/min. Expression of cell cycle-associated genes was not profoundly modulated in the kidney, in contrast to the response to acute irradiation, suggesting a threshold in the dose-rate for modulation of the expression of cell cycle-related genes in vivo following exposure to radiation. We demonstrated that changes to the gene expression profile in the testis were largely different from those in the kidney. The Gene Ontology categories “DNA metabolism”, “response to DNA damage” and “DNA replication” overlapped significantly with the clusters of genes whose expression decreased with an increase in the dose-rate to the testis. These observations provide a fundamental insight into the organ-specific responses to low-dose-rate radiation. Key Words: kidney, low-dose-rate, radiation, microarray, mitochondrial oxidative phosphorylation, testis 3 biological replicates for each experimental group were analysed. GSE14290_Kidney_raw_data.csv contains the raw data for GSM357146, GSM357425, GSM357426, GSM357452 to GSM357460. GSE14290_Testis_raw_data.csv contains the raw data for GSM357471 to GSM357482. In these files, the sample identifiers are included in the column headers.

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Expression analysis of the first-generation offspring of irradiated male mice

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