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Transcription profiling of mouse visual cortex from wild type animals sacrificed at P18 after mononuclear enucleation

ABSTRACT: Wild-type (C57Bl6) mice underwent monocular enucleation at P14 and were sacrificed 4 days later at P18. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overall Design: Gene expression in pooled samples from 5 non-deprived (ipsilateral to monocular enucleation) and 5 deprived (contralateral to monocular enucleation) visual hemispheres was compared.

ORGANISM(S): Mus musculus

SUBMITTER: Marta Majdan

PROVIDER: E-GEOD-4263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of visual experience on activity-dependent gene regulation in cortex.

Majdan Marta M Shatz Carla J CJ

Nature neuroscience 20060402 5

There are critical periods in development when sensory experience directs the maturation of synapses and circuits within neocortex. We report that the critical period in mouse visual cortex has a specific molecular logic of gene regulation. Four days of visual deprivation regulated one set of genes during the critical period, and different sets before or after. Dark rearing perturbed the regulation of these age-specific gene sets. In addition, a 'common gene set', comprised of target genes belon ...[more]

PMID: 16582906

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