Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of blasts from three APL patients expressing PML/RAR before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours

ABSTRACT: Gene expression profiles in blasts from three APL patients expressing PML/RAR were assessed before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours. We then studied a U937 clone conditionally expressing PML/RAR (U937-PR), (Grignani et al. 1993) (Alcalay et al. 2003), and compared the gene expression profile prior to and after 4 hours of treatment with 1 uM RA, to that obtained from a cell line bearing an empty vector (U937-MT). For each sample, biotinylated cRNA targets were synthesized starting from 5ug of total RNA, and hybridized to the complete set of HG-U133 Affymetrix oligonucleotide chips, which explores the expression of approximately 45,000 human transcripts. Results were analyzed using MASv5 and further elaborated with the GenePicker software. GeneChip probe sets regulated by RA in each sample were clustered into non-redundant regulated genes according to UniGene release Hs.166.

ORGANISM(S): Homo sapiens

SUBMITTER: Simone Minardi 

PROVIDER: E-MEXP-149 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Molecular signature of retinoic acid treatment in acute promyelocytic leukemia.

Meani Natalia N   Minardi Simone S   Licciulli Silvia S   Gelmetti Vania V   Coco Francesco Lo FL   Nervi Clara C   Pelicci Pier Giuseppe PG   Müller Heiko H   Alcalay Myriam M  

Oncogene 20050501 20

Acute promyelocytic leukemia (APL) is a distinct subtype of acute myeloid leukemia characterized by a block of differentiation at the promyelocytic stage. APL patients respond to pharmacological concentrations of all-trans retinoic acid (RA) and disease remission correlates with terminal differentiation of leukemic blasts. The PML/RAR oncogenic transcription factor is responsible for both the pathogenesis of APL and for its sensitivity to RA. In order to identify physiological targets of RA ther  ...[more]

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