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Deep Sequencing identifies regulated small RNAs in Dugesia japonica

ABSTRACT: We applied Illumina sequencing to identify microRNAs (miRNAs) and piwi-interacting small RNAs (piRNAs) in Dugesia japonica. Dugesia were cut up after seven day’s starvation. DJ1 is the whole Dugesia body, DJ2 is the head part, DJ3 is the tail part, DJ4 is the left part and DJ5 is the right part. Total RNA was extracted by Trizol, and preserved in ethanol, stored at -80°C until further use. 5 samples examined?DJ1,DJ2,DJ3,DJ4,DJ5. SRA study (not submitted by GEO): SRP017693

ORGANISM(S): Dugesia japonica

SUBMITTER: Maoshan Chen

PROVIDER: E-GEOD-43188 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deep sequencing identifies regulated small RNAs in Dugesia japonica.

Xu Zhenbiao Z Chen Maoshan M Ren Zhonggan Z Zhang Nian N Xu Hanmei H Liu Xiao X Tian Geng G Song Linxia L Yang Huanming H

Molecular biology reports 20130112 6

MicroRNAs (miRNAs), which are 18 ~ 24 nucleotides length, play important roles in regulating the expression of gene at the post-transcription level. Dugesia japonica is a branch of planarian organism. It is a model organism for studying the role of miRNAs in stem cell function. Next generation sequencing technology was used to identify the miRNAs of D. japonica. Bioinformatic analysis showed that 262 miRNA and miRNA* sequences were discovered, of which 102 miRNAs were the same as Schmidtea medit ...[more]

PMID: 23314792

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