Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptome profile of subcutaneous adipocytes isolated from obese vs. lean postmenopausal women

ABSTRACT: Adipocytes isolated from lean and obese postmenopausal women with no significant differences in metabolic syndrome parameters demonstrate changes in multiple inflammatory, metabolic and structural gene families. Purified adipocyte samples were isolated from subcutaneous adipose tissue surgical biopsies of 7 obese (BMI>30) and 7 lean (BMI<25) postmenoposal women and gene expression was quantified with Agilent-014850, 4X44K human whole genome platform arrays (GPL6480)

ORGANISM(S): Homo sapiens

SUBMITTER: Zheng Yin

PROVIDER: E-GEOD-44000 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation.

Deng Tuo T Lyon Christopher J CJ Minze Laurie J LJ Lin Jianxin J Zou Jia J Liu Joey Z JZ Ren Yuelan Y Yin Zheng Z Hamilton Dale J DJ Reardon Patrick R PR Sherman Vadim V Wang Helen Y HY Phillips Kevin J KJ Webb Paul P Wong Stephen T C ST Wang Rong-Fu RF Hsueh Willa A WA

Cell metabolism 20130301 3

Adipose-resident T cells (ARTs) regulate metabolic and inflammatory responses in obesity, but ART activation signals are poorly understood. Here, we describe class II major histocompatibility complex (MHCII) as an important component of high-fat-diet (HFD)-induced obesity. Microarray analysis of primary adipocytes revealed that multiple genes involved in MHCII antigen processing and presentation increased in obese women. In mice, adipocyte MHCII increased within 2 weeks on HFD, paralleling incre ...[more]

PMID: 23473035

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Project description:Background: Obesity is a risk factor for breast cancer in postmenopausal women and is associated with decreased survival and less favorable clinical characteristics such as greater tumor burden, higher grade, and poor prognosis, regardless of menopausal status. Despite the negative impact of obesity on clinical outcome, molecular mechanisms through which excess adiposity influences breast cancer etiology are not well-defined. Methods: Affymetrix U133 2.0 gene expression data were generated for 405 primary breast tumors using RNA isolated from laser microdissected tissues. Patients were classified as normal-weight (BMI<25), overweight (BMI 25-29.9) or obese (BMI>30). Statistical analysis was performed by ANOVA using Partek Genomics Suite version 6.6 using a false discovery rate <0.05 to define significance. Results: Obese patients were significantly more likely to be diagnosed >50 years or with African American ancestry compared to lean or overweight women. Pathological characteristics, including tumor stage, size or grade, lymph node status, intrinsic subtype, and breast cancer mortality did not differ significantly between groups. No significant gene expression differences were detected by BMI In a non-stratified analysis which included all subtypes or within luminal B, HER2-enriched or basal-like subtypes. Within luminal A tumors, however, 84 probes representing 77 genes from pathways such as cell cycle, p53 and mTOR signaling, tight junctions and a number of cancer associated pathways were differentially expressed. Conclusions: Identification of transcriptome differences in luminal A tumors from normal-weight compared to obese women suggests that obesity alters gene expression within ER+ tumor epithelial cells. Alterations of pathways involved in cell cycle control, tumorigenesis and metabolism may promote cellular proliferation and provide a molecular explanation for less favorable outcome of obese women with breast cancer. Targeted treatments, such as mTOR inhibitors, may allow for improved treatment and survival of obese women, especially African American women, who are more likely to be obese and suffer outcome disparities.

Dataset Information

Transcriptome profile of subcutaneous adipocytes isolated from obese vs. lean postmenopausal women

Publications

Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation.

Similar Datasets

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