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Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sandeep Wontakal

PROVIDER: E-GEOD-44148 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9.

Lu Xingwu X Wontakal Sandeep N SN Kavi Harsh H Kim Byung Ju BJ Guzzardo Paloma M PM Emelyanov Alexander V AV Xu Na N Hannon Gregory J GJ Zavadil Jiri J Fyodorov Dmitry V DV Skoultchi Arthur I AI

Science (New York, N.Y.) 20130401 6128

Eukaryotic genomes harbor transposable elements and other repetitive sequences that must be silenced. Small RNA interference pathways play a major role in their repression. Here, we reveal another mechanism for silencing these sequences in Drosophila. Depleting the linker histone H1 in vivo leads to strong activation of these elements. H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9. H1 physically interacts with Su(va ...[more]

PMID: 23559249

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Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 (Illumina smRNA-Seq]

Project description:Salivary glands or larval ovaries were isolated from transgenic flies expressing RNAi targeting Nautilus (control) or linker histone H1 using a Tub-Gal4 driver. ~200 larvae were used to isolate salivary glands or ovaries, independently. Total RNA was isolated using Trizol reagent following manufacturer's guidelines. Then 5 M-BM-5g of total RNA was separated on a polyacrylamide gel, and 18-29 nt small RNAs were isolated for cloning.

2013-04-22 | E-GEOD-44399 | biostudies-arrayexpress

Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 [Affymetrix Expression]

Project description:Indicated cells were subjected to RNAi against linker histone H1, Nautilus (control), or GFP (control). RNA was isolated and subjected to Affymetrix GeneChIP Drosophila Genome 2.0 arrays RNA was compared from cells treated with RNAi against control (Nautilus in the case of salivary glands or GFP in the case of Kc cells) and linker histone H1. Kc cells were treated once with RNAi and RNA was collected 6 days later.

2013-04-22 | E-GEOD-44398 | biostudies-arrayexpress

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Project description:modENCODE_submission_3190 This submission comes from a modENCODE project of Eric Lai. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We plan to generate a comprehensive catalog of expressed and functional microRNAs, and generate biological evidence for their regulatory activity. We plan also to delineate the primary transcription units of microRNA genes. Finally, we plan to annotate other classes of non-miRNA expressed small RNAs, as least some of which may define novel classes of small RNA genes. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: RNA-seq EXPERIMENT TYPE: RNA-seq. BIOLOGICAL SOURCE: Strain: Canton S; Tissue: Female heads; Developmental Stage: Adult Female; Genotype: wild type; Sex: Female; EXPERIMENTAL FACTORS: Strain Canton S; Tissue Female heads; Developmental Stage Adult Female

2010-10-18 | E-GEOD-24604 | biostudies-arrayexpress

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Nurf301 isoform requirements

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2010-10-17 | E-GEOD-24317 | biostudies-arrayexpress

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