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Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 (Illumina smRNA-Seq]

ABSTRACT: Salivary glands or larval ovaries were isolated from transgenic flies expressing RNAi targeting Nautilus (control) or linker histone H1 using a Tub-Gal4 driver. ~200 larvae were used to isolate salivary glands or ovaries, independently. Total RNA was isolated using Trizol reagent following manufacturer's guidelines. Then 5 M-BM-5g of total RNA was separated on a polyacrylamide gel, and 18-29 nt small RNAs were isolated for cloning.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sandeep Wontakal

PROVIDER: E-GEOD-44399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9.

Lu Xingwu X Wontakal Sandeep N SN Kavi Harsh H Kim Byung Ju BJ Guzzardo Paloma M PM Emelyanov Alexander V AV Xu Na N Hannon Gregory J GJ Zavadil Jiri J Fyodorov Dmitry V DV Skoultchi Arthur I AI

Science (New York, N.Y.) 20130401 6128

Eukaryotic genomes harbor transposable elements and other repetitive sequences that must be silenced. Small RNA interference pathways play a major role in their repression. Here, we reveal another mechanism for silencing these sequences in Drosophila. Depleting the linker histone H1 in vivo leads to strong activation of these elements. H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9. H1 physically interacts with Su(va ...[more]

PMID: 23559249

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Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1

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