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Whole genome bisulfite sequencing of rdm16ros1, ros1, nrpd1ros1 mutants and C24 wild-type

ABSTRACT: Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in rdm16ros1, ros1, nrpd1ros1 mutants and examine the effect of RDM16 on DNA methylation 4 samples examined: C24 wild type with RD29A-LUC transgene, rdm16ros1 double mutant, ros1 mutant, nrpd1ros1 mutant (all in C24 background with RD29A-LUC transgene)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jian-Kang Zhu

PROVIDER: E-GEOD-44417 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Pre-mRNA-splicing factor is required for RNA-directed DNA methylation in Arabidopsis.

Huang Chao-Feng CF Miki Daisuke D Tang Kai K Zhou Hao-Ran HR Zheng Zhimin Z Chen Wei W Ma Ze-Yang ZY Yang Lan L Zhang Heng H Liu Renyi R He Xin-Jian XJ Zhu Jian-Kang JK

PLoS genetics 20130912 9

Cytosine DNA methylation is a stable epigenetic mark that is frequently associated with the silencing of genes and transposable elements (TEs). In Arabidopsis, the establishment of DNA methylation is through the RNA-directed DNA methylation (RdDM) pathway. Here, we report the identification and characterization of RDM16, a new factor in the RdDM pathway. Mutation of RDM16 reduced the DNA methylation levels and partially released the silencing of a reporter gene as well as some endogenous genomic ...[more]

PMID: 24068953

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