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PRD1-BF1 drives VEGF-mediated innate immune suppression in tumor vasculature

ABSTRACT: Our experiments have demonstrated that VEGF signalling is a critical component of oncolytic virus infection in tumor vasculature. This effect is in part mitigated by downstream activation of the master immunoloigcal transcriptional repressor known as PRD/BF1. To investigate the role of the VEGF-PRD/BF1 signalling axis in the context of oncolytic virus infection, we isolated the RNA from sub-confluent human umbilical vein endothelial cells treated under four conditions (siRNA Control, siRNA Control + WyTK-, siRNA Control + WyTK- + VEGF165 and siRNA PRD/BF1 + WyTK- + VEGF165. Using the RNA pooled from four experiments, the goal of this study was to determine the nature & fraction of genes repressed by VEGF in the context of infection, which could be rescued by PRD/BF1.

ORGANISM(S): Homo sapiens

SUBMITTER: Cory Batenchuk

PROVIDER: E-MTAB-2963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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