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Transcription profiling of human macrophages exposed to hypoxia

ABSTRACT: The aim of this study was to investigate the effects of hypoxia on human macrophages Experiment Overall Design: Cultured macrophages from 4 donors were exposed to normoxia (21% oxygen) or hypoxia (0% oxygen) for 24 h. Two Î¼g RNA from 4 donors incubated at normoxia and 4 donors at hypoxia was pooled respectively and these 2 pools were used for the target preparation and analyzed on duplicate DNA microarrays. Comparisons were made between the expression profile from macrophages exposed to hypoxia and the expression profile from the control normoxic macrophages to identify genes regulated during hypoxia.

ORGANISM(S): Homo sapiens

SUBMITTER: Per-Arne Svensson

PROVIDER: E-GEOD-4630 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hypoxia converts human macrophages into triglyceride-loaded foam cells.

Boström Pontus P Magnusson Björn B Svensson Per-Arne PA Wiklund Olov O Borén Jan J Carlsson Lena M S LM Ståhlman Marcus M Olofsson Sven-Olof SO Hultén Lillemor Mattsson LM

Arteriosclerosis, thrombosis, and vascular biology 20060601 8

<h4>Objective</h4>Atherosclerotic lesions have regions that are hypoxic. Because the lesion contains macrophages that are loaded with lipid, we investigated whether hypoxia can influence the accumulation of lipids in these cells.<h4>Methods and results</h4>Exposure of human macrophages to hypoxia for 24 hours resulted in an increased formation of cytosolic lipid droplets and an increased accumulation of triglycerides. Exposure of the macrophages to oxidized low-density lipoprotein (oxLDL) increa ...[more]

PMID: 16741148

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