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Global gene expression profiling using total RNA [BMM, MPI and SPG]

ABSTRACT: Differentiation of myeloid progenitor cells leads to distinct populations of mononuclear phagocytes.Various macrophages exhibit distinct biological properties. Here wanted to delineate and characterize gene expression patterns in bone marrow derived macrophages Here wanted to delineate and characterize gene expression patterns in two newly established, self-renewing, in vitro grown non-transformed lines (MPI cells) and in the SP37A3 immature myeloid dendritic cell line. We used microarrays to detail the global programme of gene expression in MPI cells and to compare them to other types of mononuclear phagocytes. [BMM] Total cellular RNA from ex vitro differentiated bone marrow derived macrophages was extracted and hybridized to Affymetrix microarrays to carry out cluster analysis and overlap and gene set analysis for enriched pathways. [MPI and SPG] Total cellular RNA from independently established MPI lines and a splenic immature dendritic cell line was extracted and hybridized to Affymetrix microarrays to carry out cluster analysis and overlap and gene set analysis for enriched pathways.

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Diehl

PROVIDER: E-GEOD-47473 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global gene expression profiling using total RNA [BMM, MPI and SPG]

Project description:Differentiation of myeloid progenitor cells leads to distinct populations of mononuclear phagocytes.Various macrophages exhibit distinct biological properties. Here wanted to delineate and characterize gene expression patterns in bone marrow derived macrophages Here wanted to delineate and characterize gene expression patterns in two newly established, self-renewing, in vitro grown non-transformed lines (MPI cells) and in the SP37A3 immature myeloid dendritic cell line. We used microarrays to detail the global programme of gene expression in MPI cells and to compare them to other types of mononuclear phagocytes.

2013-06-08 | GSE47473 | GEO

Global gene expression profiling using newly synthesized RNA [mock and LPS]

Project description:Innate stimulation with TLR ligands leads to the activation of various genes in macrophages and various populations of these cells may exhibit different responses. Here wanted to delineate and characterize these transcriptional responses in newly established, self-renewing, in vitro grown non-transformed lines (MPI cells) and bone marrow derived macrophages We used microarrays to detail the global programme of gene expression Newly synthesized RNA from independently established MPI lines and bone marrow derived macrophages was extracted and hybridized to Affymetrix microarrays

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Global gene expression profiling using newly synthesized RNA [mock and LPS]

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Licensing of PPARg-regulated gene expression by IL-4-induced alternative macrophage activation

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2010-11-18 | E-GEOD-16387 | biostudies-arrayexpress

Changes in glycogene expression during maturation of dendritic cells

Project description:To investigate the expression of glycosyltransferases in dendritic cells and the changes in expression associated with maturation. Healthy normal donor RNA preparations from Resting macrophages (Macro), LPS-mature dendritic cells (LPSMDC), IL-15 immature dendritic cells (IL15DC), Dexamethasone immature dendritic cells (DxMDC) from 2 different donors.

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Next Generation Sequencing Facilitates Quantitative Analysis of dendritic cell Transcriptomes during LPS response

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse GM-CSF and IL-4, immature DCs were obtained before or after LPS stimulation. Immature DCs were sorted respectively based on marker CD86 and Iab(MHCII) using flowcytrometer. DC mRNA profiles were generated by deep sequencing,using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified hundreds of genes with significant mRNA variation during LPS stimulation. DC mRNA profiles immature BMDCs were generated by deep sequencing

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RNA Sequencing Analysis of Mouse Bone Marrow Derived Dendritic Cells Transcriptomes During Maturation

Project description:Purpose:The purpose of this study is to detect activated or silenced genes during LPS-induced dendritic cell maturation. Gene expression differences between two samples could be found using transcriptome profiling (RNA-seq) analysis. Methods:Mouse dendritic cells were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse GM-CSF and IL-4, mature DCs were obtained after LPS induced maturation. Immature DCs and mature DCs were sorted respectively based on maturation marker CD86 and Iab(MHCII) using flowcytrometer. DC mRNA profiles were generated by deep sequencing,using Illumina Results: We mapped about 10 million sequence reads per sample to the mouse genome, identified 1,300 upregulated genes and 1,475 dow regulated genes during dendritic cell maturation. DC mRNA profiles immature and mature moouse BMDCs were generated by deep sequencing

2015-03-04 | E-GEOD-60307 | biostudies-arrayexpress

A miR-155-ruled microRNA hierarchy in dendritic cell maturation and macrophage activation

Project description:MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute (Ago) protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. MiRNA expression can be controlled by transcription factors and can therefore be cell type- or tissue-specific. Here we have analyzed miRNA expression profiles in murine monocyte-derived dendritic cells (DCs) and macrophages upon stimulation with LPS, LDL, eLDL and oxLDL to identify not only stimuli-specific miRNA, but also to identify a hierarchical miRNA system involving miR-155. For this, miR-155 knockout dendritic cells and macrophages were also sequenced using the same stimuli. Sequencing of murine monocyte-derived dendritic cells and macrophages (each wild type and miR-155 knock out cells) matured and stimulated, respectively, by LPS, oxLDL, eLDL or LDL.

2014-01-03 | E-GEOD-48404 | biostudies-arrayexpress

Real-time quantitative PCR analysis of human dendritic cells

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2011-05-08 | E-GEOD-29131 | biostudies-arrayexpress

Imbalance of Ly6Chi and Ly6Clo monocytes/macrophages worsens hyperoxia-induced lung injury and is rescued by IFN-gamma

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