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SMORE-seq maps both ends of transcripts and identifies widespread promoter-associated non-coding RNA governed by TATA elements.

ABSTRACT: SMORE-seq (Simultaneous Mapping Of RNA Ends by sequencing) is developted to simultaneously identify the strongest TSS (Transcription Start Site) and PAS (Polyadenylation Site) SMORE-seq were performed in wild type Saccharomyces cerevisiae

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Vishy Iyer

PROVIDER: E-GEOD-49026 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Simultaneous mapping of transcript ends at single-nucleotide resolution and identification of widespread promoter-associated non-coding RNA governed by TATA elements.

Park Daechan D Morris Adam R AR Battenhouse Anna A Iyer Vishwanath R VR

Nucleic acids research 20140110 6

Understanding the relationships between regulatory factor binding, chromatin structure, cis-regulatory elements and RNA-regulation mechanisms relies on accurate information about transcription start sites (TSS) and polyadenylation sites (PAS). Although several approaches have identified transcript ends in yeast, limitations of resolution and coverage have remained, and definitive identification of TSS and PAS with single-nucleotide resolution has not yet been achieved. We developed SMORE-seq (si ...[more]

PMID: 24413663

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