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Integrated expression profiles of mRNA and miRNA in polarized primary murine microglia (mRNA)

ABSTRACT: The aim of this study was to determine the role that miRNAs have on influencing murine microgial phenotypes under M1(LPS) and M2a (IL-4) stimulating conditions. This Series includes expression data obtained from mRNA gene expression microarrays; miRNA expression profiles obtained from the same RNA samples were deposited as a separate Series. 9 total samples were analyzed, n=3 for the three stimulating conditions (resting state, LPS and IL-4) for murine primary microglia. This allowed us to perform the following pairwise comparisons: PBS vs. LPS, and PBS vs. IL-4. This was performed for both mRNA and miRNA gene expression profiles.

ORGANISM(S): Mus musculus

SUBMITTER: Robert Freilich

PROVIDER: E-GEOD-49329 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrated expression profiles of mRNA and miRNA in polarized primary murine microglia.

Freilich Robert W RW Woodbury Maya E ME Ikezu Tsuneya T

PloS one 20131111 11

Neuroinflammation contributes to many neurologic disorders including Alzheimer's disease, multiple sclerosis, and stroke. Microglia is brain resident myeloid cells and have emerged as a key driver of the neuroinflammatory responses. MicroRNAs (miRNAs) provide a novel layer of gene regulation and play a critical role in regulating the inflammatory response of peripheral macrophages. However, little is known about the miRNA in inflammatory activation of microglia. To elucidate the role that miRNAs ...[more]

PMID: 24244499

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Integrated expression profiles of mRNA and miRNA in polarized primary murine microglia (miRNA)

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