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Expression analysis of YAP and Angiomotin target genes

ABSTRACT: To assess changes in gene expression regulated by Yap1 or Angiomotin, either Yap1 or Angiomotin were knocked down in HEK293 cells using shRNA and expression was assessed by microarray analysis and compared to expression patterns of wildtype cells. Microarray analysis was performed to compare gene expression in Amot-KD, Yap-KD, and control HEK293 cells. Samples from three individual experiments for each of the three groups were analyzed.

ORGANISM(S): Homo sapiens

SUBMITTER: Louise Showe

PROVIDER: E-GEOD-49406 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The p130 isoform of angiomotin is required for Yap-mediated hepatic epithelial cell proliferation and tumorigenesis.

Yi Chunling C Shen Zhewei Z Stemmer-Rachamimov Anat A Dawany Noor N Troutman Scott S Showe Louise C LC Liu Qin Q Shimono Akihiko A Sudol Marius M Holmgren Lars L Stanger Ben Z BZ Kissil Joseph L JL

Science signaling 20130903 291

The Hippo-Yap signaling pathway regulates a number of developmental and adult cellular processes, including cell fate determination, tissue growth, and tumorigenesis. Members of the scaffold protein angiomotin (Amot) family interact with several Hippo pathway components, including Yap (Yes-associated protein), and either stimulate or inhibit Yap activity. We used a combination of genetic, biochemical, and transcriptional approaches to assess the functional consequences of the Amot-Yap interactio ...[more]

PMID: 24003254

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