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Genome wide DNA methylation analysis of leukemia and reprogrammed leukemia cells

ABSTRACT: We apply cellular reprogramming to an induced pluripotent cell state to human cell lines and primary samples exhibiting Chronic Myoloid Leukemia (CML). Addtionally, we generated an inducible CML BCR-ABL transgenic mouse model. Biological replicates (duplicates) were available for all samples. DNA methylation profiles for all cells were generated using Reduced Representation Bisulfite Sequencing (RRBS). DNA methylation profiling of human leukemia cell lines, primary cells, derived iPS cells as well as transgenic mouse models for CML in duplicates using RRBS.

ORGANISM(S): Homo sapiens

SUBMITTER: Fabian Müller

PROVIDER: E-GEOD-50456 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dissecting the role of aberrant DNA methylation in human leukaemia.

Amabile Giovanni G Di Ruscio Annalisa A Müller Fabian F Welner Robert S RS Yang Henry H Ebralidze Alexander K AK Zhang Hong H Levantini Elena E Qi Lihua L Martinelli Giovanni G Brummelkamp Thijn T Le Beau Michelle M MM Figueroa Maria E ME Bock Christoph C Tenen Daniel G DG

Nature communications 20150522

Chronic myeloid leukaemia (CML) is a myeloproliferative disorder characterized by the genetic translocation t(9;22)(q34;q11.2) encoding for the BCR-ABL fusion oncogene. However, many molecular mechanisms of the disease progression still remain poorly understood. A growing body of evidence suggests that the epigenetic abnormalities are involved in tyrosine kinase resistance in CML, leading to leukaemic clone escape and disease propagation. Here we show that, by applying cellular reprogramming to ...[more]

PMID: 25997600

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