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Differential methylation between mouse embyronic stem cell states

ABSTRACT: We generated RRBS data between Rex1-high and Rex1-low cells to identify differentially methylated promoters. We observe bimodal methylation levels in both subpopulations, and that Rex1-low cells accumulate increased levels of methylation, frequently as a function of local CpG density We sorted Rex1 high and low cells using a GFP-knockin reporter cell line (kindly provided by Austin Smith), and performed RRBS on the subpopulations

ORGANISM(S): Mus musculus

SUBMITTER: Michael Elowitz

PROVIDER: E-GEOD-58396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dynamic heterogeneity and DNA methylation in embryonic stem cells.

Singer Zakary S ZS Yong John J Tischler Julia J Hackett Jamie A JA Altinok Alphan A Surani M Azim MA Cai Long L Elowitz Michael B MB

Molecular cell 20140701 2

Cell populations can be strikingly heterogeneous, composed of multiple cellular states, each exhibiting stochastic noise in its gene expression. A major challenge is to disentangle these two types of variability and to understand the dynamic processes and mechanisms that control them. Embryonic stem cells (ESCs) provide an ideal model system to address this issue because they exhibit heterogeneous and dynamic expression of functionally important regulatory factors. We analyzed gene expression in ...[more]

PMID: 25038413

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2014-03-28 | E-GEOD-56310 | biostudies-arrayexpress

Fundamental differences in promoter CpG island DNA hypermethylation between human cancer and genetically engineered mouse models of cancer [RRBS]

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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