Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Normal vs. Tumor Prostate miRNA

ABSTRACT: Comparison of miRNA expression profiles in a small set of prostate needle core biopsies or fine needle aspirates. Keywords: Expression profiling of prostate needle core biopsies MicroRNA expression was compared between a pooled normal sample consisting of 10 separate normal adjacent to tumor prostate needle core biopsies, two prostate tumor cell lines (PC3 and LNCaP), two needle core biopsies, and a fine needle aspirate of a prostate tumor metastasis to the supraclavicular lymph node. MicroRNA was isolated from fresh frozen tissue sections of the needle core biopsies using the mirVana miRNA Isolation kit from Ambion per the manufacturer's instructions. MicroRNA was amplified using 10 ng input and 750 ng of amplified material was subsequently labeled for hybridization. All samples were normalized to the same normal prostate control.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Mattie

PROVIDER: E-GEOD-4574 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies.

Mattie Michael D MD Benz Christopher C CC Bowers Jessica J Sensinger Kelly K Wong Linda L Scott Gary K GK Fedele Vita V Ginzinger David D Getts Robert R Haqq Chris C

Molecular cancer 20060619

<h4>Background</h4>Recent studies indicate that microRNAs (miRNAs) are mechanistically involved in the development of various human malignancies, suggesting that they represent a promising new class of cancer biomarkers. However, previously reported methods for measuring miRNA expression consume large amounts of tissue, prohibiting high-throughput miRNA profiling from typically small clinical samples such as excision or core needle biopsies of breast or prostate cancer. Here we describe a novel ...[more]

PMID: 16784538

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Dataset Information

Normal vs. Tumor Prostate miRNA

Publications

Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies.

Similar Datasets

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