Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

47 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

S. cerevisiae mutant with a constitutively activated Ras/cAMP pathway

ABSTRACT: Comparison of the transcriptomes of Saccharomyces cerevisiae wild type FY23 and a PDE2 deletion mutant DJ28. Keywords = PDE2 Keywords = Ras/cAMP pathway

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Dawn Jones

PROVIDER: E-GEOD-600 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Transcriptome profiling of a Saccharomyces cerevisiae mutant with a constitutively activated Ras/cAMP pathway.

Jones D L DL Petty J J Hoyle D C DC Hayes A A Ragni E E Popolo L L Oliver S G SG Stateva L I LI

Physiological genomics 20031216 1

Often changes in gene expression levels have been considered significant only when above/below some arbitrarily chosen threshold. We investigated the effect of applying a purely statistical approach to microarray analysis and demonstrated that small changes in gene expression have biological significance. Whole genome microarray analysis of a pde2Delta mutant, constructed in the Saccharomyces cerevisiae reference strain FY23, revealed altered expression of approximately 11% of protein encoding g ...[more]

PMID: 14570984

Similar Datasets

New targets of Drosophila adenosine deaminase acting on RNA (dADAR)

Project description:The aim of this experiment is to identify new targets of Drosophila adenosine deaminase acting on RNA (dADAR) by enriching Inosine-containing mRNA (I-mRNA) from wild type (C-S) and dADAR mutant flies and then hybridization with Drosophila cDNA arrays.

2010-06-05 | E-GEOD-529 | biostudies-arrayexpress

Heterogous hybridization experiment

Project description:Fish and Chips: Expression Profiling in Non-traditional Model Systems Using a Cichlid Fish cDNA Microarray This series represents the 26 arrays that went into Renn et al 2004 (submitted January 16th), and one additional hybridization (GSM15240) that was not included in the publication analysis for statistical reasons.

2010-06-09 | E-GEOD-975 | biostudies-arrayexpress

cDNA array 6800 genes (NIB) - HaCaT J10 24h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 24 h after irradiation. Keywords = human keratinocytes, gamma irrdiation

2003-03-25 | E-GEOD-356 | biostudies-arrayexpress

cDNA array 2304 genes (OSPE) - HaCaT J10 24h - 0.5& 2 Gy

Project description:Human keratinocytes were cultured to confluence (day 10). Differentiated confluent cells were irradiated at 0.5 and 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap procedure 24 h after irradiation. Keywords = Human keratinocytes, gamma irradiation

2010-10-08 | E-GEOD-354 | biostudies-arrayexpress

Cross species comparison of expression patterns

Project description:Comparison of expression in liver samples of human, chimp, orang and rhesus, by using a novel multi-species cDNA array

2010-06-10 | E-GEOD-2009 | biostudies-arrayexpress

Life cycle of Drosophila melanogaster

Project description:Molecular genetic studies of Drosophila melanogaster have led to profound advances in understanding the regulation of development. Here we report gene expression patterns for nearly one-third of all Drosophila genes during a complete time course of development. Mutations that eliminate eye or germline tissue were used to further analyze tissue-specific gene expression programs. These studies define major characteristics of the transcriptional programs that underlie the life cycle, compare development in males and females, and show that large-scale gene expression data collected from whole animals can be used to identify genes expressed in particular tissues and organs or genes involved in specific biological and biochemical processes.

2003-01-05 | E-GEOD-94 | biostudies-arrayexpress

MES-4, an autosome-associated HMT that participates in silencing the X chromosomes in the C. elegans germ line

Project description:Microarray-based expression profiling of dissected gonads from mes-4 mutants reveals that MES-4 is required to repress expression of a set of X-linked genes. We isolated dissected gonads from mes-4(bn85) mutant adults and compared to dissected gonads from wild type (N2 animals. RNA was linearly amplified prior to labeling for all genotypes. Comparison was done in quadruplicate on independently grown and isolated animals.

2010-07-01 | E-GEOD-5454 | biostudies-arrayexpress

Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1(E2F)does not require LIN-35(pRB)

Project description:Microarray-based expression profiling of dissected gonads from efl-1, dpl-1 and lin-35 mutants reveals that EFL-1 and DPL-1 promote expression of an extensively overlapping set of target genes, consistent with the expectation that these two proteins function as a heterodimer. Regulatory regions upstream of many of these target genes have a canonical E2F binding site, suggesting that their regulation by EFL-1/DPL-1 is direct. Many EFL-1/DPL-1 responsive genes encode proteins required for oogenesis and early embryogenesis, rather than cell cycle components. By contrast, LIN-35 appears to function primarily as a repressor of gene expression in the germ line, and the genes that it acts on are for the most part distinct from those regulated by EFL-1 and/or DPL-1 We isolated dissected gonads from dpl-1, efl-1 and lin-35 mutant adults and compared each to control dissected gonads. RNA was linearly amplified prior to labeling for all genotypes. Each comparison was done in triplicate (dpl-1 and lin-35) or in quadruplicate (efl-1) on independently grown and isolated animals.

2010-07-01 | E-GEOD-5071 | biostudies-arrayexpress

Porcine liver

Project description:Fasting, fed, and Clofibric Acid comparisons

2003-11-14 | E-GEOD-714 | biostudies-arrayexpress

HaCaT keratinocytes day3 vs HaCaT keratinocytes day10

Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes

2003-03-10 | E-GEOD-336 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data