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Transcription profiling of gulahuman CD8 T lyphocytes overexpressing hTERT to investigate regulation of profileration

ABSTRACT: Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD2 was profiled on U133Plus 2.0 and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 chip (hTERT_YOUNG_HD1_REP2) excluded because of low quality hybridization.

ORGANISM(S): Homo sapiens

SUBMITTER: Mauro Delorenzi

PROVIDER: E-GEOD-5142 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mechanisms regulating the proliferative potential of human CD8+ T lymphocytes overexpressing telomerase.

Menzel Olivier O Migliaccio Marco M Goldstein Darlene R DR Dahoun Sophie S Delorenzi Mauro M Rufer Nathalie N

Journal of immunology (Baltimore, Md. : 1950) 20060901 6

In human somatic cells, including T lymphocytes, telomeres progressively shorten with each cell division, eventually leading to a state of cellular senescence. Ectopic expression of telomerase results in the extension of their replicative life spans without inducing changes associated with transformation. However, it is yet unknown whether somatic cells that overexpress telomerase are physiologically indistinguishable from normal cells. Using CD8+ T lymphocyte clones overexpressing telomerase, w ...[more]

PMID: 16951325

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Transcription profiling of human CD* T lymphocytes overexpressing hTERT

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Transcription profiling of human CD8 T lymphocytes from two healthy donors transduced with hTERT

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Project description:Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD2 was profiled on U133Plus 2.0 and submitted as a separate GEO series. Keywords: Cell Line Comparison.

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Project description:Expression in GFP vs. GFP/hTERT transduced CD8 T Lymphocytes from Healty Donors (HD) 1 and 2 at early and late passages. Using CD8+ T lymphocyte clones over-expressing telomerase we investigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. Keywords: Cell Line Comparison.

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Homo sapiens

Project description:hTERT effects in CD8 T Lymphocytes HD2

| PRJNA96657 | ENA

Homo sapiens

Project description:hTERT effects in CD8 T Lymphocytes HD1

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