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Transcription profiling of human CD* T lymphocytes overexpressing hTERT

ABSTRACT: Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD1 was profiled on U133A and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 condition (GFP YOUNG) lost because of problems in cell culture.

ORGANISM(S): Homo sapiens

SUBMITTER: Mauro Delorenzi

PROVIDER: E-GEOD-5143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mechanisms regulating the proliferative potential of human CD8+ T lymphocytes overexpressing telomerase.

Menzel Olivier O Migliaccio Marco M Goldstein Darlene R DR Dahoun Sophie S Delorenzi Mauro M Rufer Nathalie N

Journal of immunology (Baltimore, Md. : 1950) 20060901 6

In human somatic cells, including T lymphocytes, telomeres progressively shorten with each cell division, eventually leading to a state of cellular senescence. Ectopic expression of telomerase results in the extension of their replicative life spans without inducing changes associated with transformation. However, it is yet unknown whether somatic cells that overexpress telomerase are physiologically indistinguishable from normal cells. Using CD8+ T lymphocyte clones overexpressing telomerase, w ...[more]

PMID: 16951325

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