Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HTERT effects in CD8 T Lymphocytes HD2

ABSTRACT: Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD1 was profiled on U133A and submitted as a separate GEO series. Keywords: Cell Line Comparison.

ORGANISM(S): Homo sapiens

PROVIDER: GSE5143 | GEO | 2006/06/24

SECONDARY ACCESSION(S): PRJNA96657

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of human CD* T lymphocytes overexpressing hTERT

Project description:Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD1 was profiled on U133A and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 condition (GFP YOUNG) lost because of problems in cell culture.

2008-06-13 | E-GEOD-5143 | biostudies-arrayexpress

hTERT effects in CD8 T Lymphocytes HD1

Project description:Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD2 was profiled on U133Plus 2.0 and submitted as a separate GEO series. Keywords: Cell Line Comparison.

2006-06-24 | GSE5142 | GEO

hTERT effects in CD8 T Lymphocytes from two healthy donors

Project description:Expression in GFP vs. GFP/hTERT transduced CD8 T Lymphocytes from Healty Donors (HD) 1 and 2 at early and late passages. Using CD8+ T lymphocyte clones over-expressing telomerase we investigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. Keywords: Cell Line Comparison.

2006-06-24 | GSE5144 | GEO

Transcription profiling of gulahuman CD8 T lyphocytes overexpressing hTERT to investigate regulation of profileration

Project description:Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD2 was profiled on U133Plus 2.0 and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 chip (hTERT_YOUNG_HD1_REP2) excluded because of low quality hybridization.

2008-06-13 | E-GEOD-5142 | biostudies-arrayexpress

Transcription profiling of human CD8 T lymphocytes from two healthy donors transduced with hTERT

Project description:Expression in GFP vs. GFP/hTERT transduced CD8 T Lymphocytes from Healty Donors (HD) 1 and 2 at early and late passages. Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. Experiment Overall Design: triplicates of 4 conditions for each of 2 donors, 1 chip (hTERT_YOUNG_HD1_REP2) of the first donor excluded because of low quality hybridization, one condition (GFP YOUNG)lost from second donor because of problems in cell culture.

2008-06-13 | E-GEOD-5144 | biostudies-arrayexpress

Homo sapiens

Project description:hTERT effects in CD8 T Lymphocytes HD2

| PRJNA96657 | ENA

Transcriptome data from U2OS cells and hTERT CRISPR clones

Project description:To assess the biological significances of p-hTERT in ALT cells, we used the CRISPR-Cas9 system targeting the hTERT gene and performed RNA-seq to analyze the profile of gene expression in hTERT-CRISPR clones (clone#1 and #2). The subsequent gene ontology (GO) analysis showed enrichment of genes involved in cell cycle process and DNA replication within genes down-regulated in hTERT-CRISPR clones.

2024-04-08 | GSE226963 | GEO

Homo sapiens

Project description:hTERT effects in CD8 T Lymphocytes HD1

| PRJNA96655 | ENA

U2OS cells and hTERT CRISPR clones

Project description:U2OS cells and hTERT CRISPR clones

| PRJNA942321 | ENA

Gene expression in hTERT-RPE1 cells with overexpression of MFRP

Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion.

2014-03-01 | GSE44540 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data