Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of 2 cell mouse embryos of BRG1 mice and wild type mice to understand the effects of BRG1 on zygotic genome activation.

ABSTRACT: Embryos were collected at 2 cell stage. cRNA from four biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430 v2. Experiment Overall Design: 4 embryo at 2 cell stage biological replicates were analyzed.

ORGANISM(S): Mus musculus

SUBMITTER: Hua Pan

PROVIDER: E-GEOD-5371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Maternal BRG1 regulates zygotic genome activation in the mouse.

Bultman Scott J SJ Gebuhr Thomas C TC Pan Hua H Svoboda Petr P Schultz Richard M RM Magnuson Terry T

Genes & development 20060701 13

Zygotic genome activation (ZGA) is a nuclear reprogramming event that transforms the genome from transcriptional quiescence at fertilization to robust transcriptional activity shortly thereafter. The ensuing gene expression profile in the cleavage-stage embryo establishes totipotency and is required for further development. Although little is known about the molecular basis of ZGA, oocyte-derived mRNAs and proteins that alter chromatin structure are likely crucial. To test this hypothesis, we ge ...[more]

PMID: 16818606

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Project description:E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells; In the study we demonstrated shared gene expression in embryonic heart valve development and Osteoblast progenitor cells. The atrioventricular (AV) valves of the heart develop from undifferentiated mesenchymal endocardial cushions, that later remodel into stratified valves with diversified extracellular matrix (ECM). Because the mature valves express genes associated with osteogenesis and exhibit disease-associated calcification, we hypothesized the existence of shared regulatory pathways active in the remodeling AV valves and in bone progenitor cells. In order to define gene regulatory programs of valvulogenesis relative to osteoblast progenitors, we undertook Affymetrix gene expression profiling analysis of murine embryonic day (E)12.5 AV cushions compared to E17.5 remodeled AV valves (mitral and tri-cuspid) and to pre-osteoblast MC3T3-E1 (subclone4) cells. Overall MC3T3 cells were significantly more similar to E17.5 valves than to E12.5 cushions, supporting the hypothesis that valve remodeling involves the expression of many genes also expressed in osteoblasts. Several transcription factors characteristic of mesenchymal and osteoblast precursor cells, including Twist1 are predominant in E12.5 cushion. Valve remodeling also includes differential regulation of matrix metalloproteinases and their inhibitors as well as characteristic collagen isoform switching. Among the most highly enriched genes during valvulogenesis were members of the small leucine-rich proteoglycan (SLRP) family including Asporin, a known negative regulator of osteoblast differentiation and mineralization. Together, these data support shared gene expression profiles of the remodeling valves and osteoblast bone precursor cells in normal valve development and homeostasis with potential functions in calcific valve disease. Experiment Overall Design: In the study, we hybridized RNA from E12.5 AV cushion and E17.5 AV valve from wild-type FVB/N mice and in vitro cultured MC3T3 cells to Affymetrix MOE430 2 GeneChipÂ® arrays.

Dataset Information

Transcription profiling of 2 cell mouse embryos of BRG1 mice and wild type mice to understand the effects of BRG1 on zygotic genome activation.

Publications

Maternal BRG1 regulates zygotic genome activation in the mouse.

Similar Datasets

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