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Contribution of the scavenger receptor MARCO to TLR-induced activation of dendritic cells

ABSTRACT: To gain additional insight into the role of MARCO in dendritic cell activation and function, we profiled transcriptomes of mouse splenic dendritic cells obtained from MARCO deficient mice and their wild type counterparts under resting and activating conditions. Pure dendritic cells from the spleens of WT and MARCO KO mice were cultured overnight in PBS or Lipopolysaccharide (LPS). Gene expression profiling was performed using Affymetrix platform.

ORGANISM(S): Mus musculus

SUBMITTER: M. Simo Arredouani

PROVIDER: E-GEOD-55068 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Contribution of the scavenger receptor MARCO to TLR-induced activation of dendritic cells

Project description:To gain additional insight into the role of MARCO in dendritic cell activation and function, we profiled transcriptomes of mouse splenic dendritic cells obtained from MARCO deficient mice and their wild type counterparts under resting and activating conditions.

2014-02-18 | GSE55068 | GEO

Role of IRAKM in IL-33-induced dendritic cell activation

Project description:To gain additional insight into the role of IRAKM in dendritic cell activation in response to IL-33 challenge, we profiled transcriptomes of mouse DC2.4 dendritic cell lines with or without IRAKM following IL-33 treatment in vitro. IRAKM-sufficient and deficient DC2.4 cells were cultured overnight in the absence or presence of IL-33. Gene expression profiling was performed using Affymetrix platform.

2015-03-03 | E-GEOD-66431 | biostudies-arrayexpress

Effect of LMP7 and MECL1-immunoproteasome subunits deficiency on the transcriptome of mouse bone marrow-derived dendritic cells

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2014-07-02 | E-GEOD-52616 | biostudies-arrayexpress

Transcription profiling by array of human dendritic cells co-cultured with carcinoma cells or treated with lipopolysaccharide

Project description:Human dendritic cells were co-cultured with carcinoma cells (A549 or SK-MES-1) or were treated with lipopolysaccharide (LPS).<br>There were three biological replicates of each condition plus untreated controls giving 12 samples and microarrays in total.<br>Samples were processed with standard Affymetrix IVT protocols and hybridised to Affymetrix Human Genome U133 GeneChips. Data were processed in Bioconductor using RMA.

2009-02-27 | E-MEXP-1958 | biostudies-arrayexpress

Gene expression of distinct lung dendritic cell subsets

Project description:Pulmonary dendritic cells are heterogenous cells comprise four distinct subsets including two conventional dendritic cell subsets, CD103+ and CD11bhiCD14lo cells, and two monocyte-derived dendritic cell subsets. Their functions in terms of migration and T cell activation are distinct, but genes regulating their features are to be determined. We used microarrays to identify a select set of genes that are expressed in conventinal dendritic cells and in monocyte-derived dendriti cells. Four distinct lung DC subsets were purified by flow cytometry-based sorting after inhalation of lipopolusaccharide and ovalbumin. Each subset has three replicates.

2015-04-02 | E-GEOD-64896 | biostudies-arrayexpress

Recombinant and plasma-derived Factor VIII products induce distinct splenic cytokine microenvironments in hemophilia A

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2010-05-19 | E-GEOD-15809 | biostudies-arrayexpress

Transcriptome analysis of primary human monocyte differentiation

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Vitamin D modulation of innate immune signalling in Dendritic Cells

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2016-10-03 | E-MTAB-4883 | biostudies-arrayexpress

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2012-09-30 | E-GEOD-40647 | biostudies-arrayexpress

Gene expression in dendritic cells stimulated with LPS in conditions allowing or inhibiting NFAT activation

Project description:Interleukin-2 (IL-2) is one of the molecules produced by mouse dendritic cells (DCs) after stimulation by Toll like receptor (TLR) agonists. By analogy with the events following T-cell receptor (TCR) engagement leading to IL-2 production we have observed that DC stimulation with lipopolysaccharide (LPS) induces Src-family kinase and phospholipase C (PLC)Î³2 activation, influx of extracellular Ca2+ and calcineurin-dependent nuclear NFAT translocation. We have also observed that the initiation of this pathway is independent of TLR4 engagement, and dependent exclusively on CD14. To determine the role of NFAT in LPS activated dendritic cells we have performed microarray analysis in conditions allowing or inhibiting NFAT activation. We show here that LPS-induced NFAT activation via CD14 is necessary to cause death of terminally differentiated DCs, an event that is essential for maintaining self-tolerance and preventing autoimmunity. Consequently, blocking this pathway in vivo causes prolonged DC survival and an increase in T cell priming capability. Gene expression analyses were performed using Affymetrix GeneChips in the following groups of murine bone marrow-derived dendritic cells: 1) CD14-deficient BMDCs stimulated with LPS; 2) wtBMDCs stimulated with LPS in presence of EGTA; 3) wtBMDCs stimulated with LPS. This experimental setting allowed us to select for effects due to Ca2+ fluxes and exclude the effects due to other causes, particularly the block of TRIF recruitment in CD14-deficient cells and the EGTA effects unrelated to Ca2+ chelation.

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