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Expression of mRNAs in 230-238 B-cell line infected with a plasmid encoding the GFP protein or a plasmid encoding both the GFP and FOG1 proteins

ABSTRACT: The overexpression of Friend Of GATA1 (FOG1) in early B-cell stages may promote the proliferation of B-cells by changing the expression level of a certain number of key genes involved in cell cycle control. The 230-238 cell line was either infected with a plasmid encoding the GFP protein or a plasmid encoding both the GFP and FOG1 proteins. After infection, cells were put in culture in RPMI1640 medium for 3 days before to be sorted for being GFP+. They were put back again in culture for 4 days more after the sort. Then, RNA isolation was performed using the following QIAGEN kit: RNeasy micro without DNase treatment.

ORGANISM(S): Mus musculus

SUBMITTER: Tim Roloff

PROVIDER: E-GEOD-55498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of mRNAs in 230-238 B-cell line infected with a plasmid encoding the GFP protein or a plasmid encoding both the GFP and FOG1 proteins

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