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Stress-induced signaling to the nucleosome in serum deprived mouse fibroblasts.

ABSTRACT: We have employed chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze changes in chromatin architecture as well as the occupancy of two RNA Polymerase II (RNAPII) isoforms, initiation-competent (RNAPIIS5ph) as well as elongation-competent (RNAPIIS2ph) upon stress induction. We used resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin to induce the p38/MAP kinase pathway. Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 1 h (in duplicates). Untreated, serum-starved cells were used as a control. Isolated chromatin was subjected to immunoprecipitation with the following antibodies: a-H3S28ph, a-H3K9ac, a-H3K4me3, a-RNAPIIS5ph and a-RNAPIIS2ph. Resulting DNA was sequenced using Illumina platforms.

ORGANISM(S): Mus musculus

SUBMITTER: Anna Sawicka

PROVIDER: E-GEOD-55774 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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H3S28 phosphorylation is a hallmark of the transcriptional response to cellular stress.

Sawicka Anna A Hartl Dominik D Goiser Malgorzata M Pusch Oliver O Stocsits Roman R RR Tamir Ido M IM Mechtler Karl K Seiser Christian C

Genome research 20140818 11

The selectivity of transcriptional responses to extracellular cues is reflected by the deposition of stimulus-specific chromatin marks. Although histone H3 phosphorylation is a target of numerous signaling pathways, its role in transcriptional regulation remains poorly understood. Here, for the first time, we report a genome-wide analysis of H3S28 phosphorylation in a mammalian system in the context of stress signaling. We found that this mark targets as many as 50% of all stress-induced genes, ...[more]

PMID: 25135956

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