Loss of Runx3 in osteoblasts provokes severe congenital osteopenia
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ABSTRACT: Murine Runx3 is expressed in developing bone osteoblasts (OBLs) and its deletion in these cells culminates in severe congenital osteopenia. We demonstrate that Runx3 is non-redundantly involved in the proliferation of early pre-committed OBL progenitor cells, a critical step in the generation of adequate numbers of bone-forming OBLs. Thus, in the absence of Runx3 in cells of this lineage, the number of mature/active OBLs is significantly diminished, providing a mechanistic explanation to the observed osteopenia. This experiment was aimed at identifying genes that are likely regulated by Runx3 along osteoblastogenesis. To elucidate the Runx3-mediated transcriptional program along OBL differentiation, we compared gene expression profile in WT and Runx3f/f/Col1a1-cre calvarial OBLs. Progenitor cells were purified and RNA extracted, reverse-transcribed, fragmented, labeled and hybridized to GeneChip® Mouse Gene 2.0 ST Array.
ORGANISM(S): Mus musculus
SUBMITTER: Omri Bauer
PROVIDER: E-GEOD-57195 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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