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RNA expression profiling of human mPB or CB-derived CD34+ cells treated with UM171 at different doses

ABSTRACT: RNASeq data for mPB or CB-derived CD34+ exposed to UM171 human mobilized peripheral blood or cord blood-derived CD34(+) cells were cultured for 16 hours with vehicle (DMSO), dose response of UM171 [11.9nM, 19nM, 30.5nM, 48.8nM, 78.1nM and 125nM], SR1 [500nM] and combination of( UM171 [48.8nM]+SR1 [500nM])

ORGANISM(S): Homo sapiens

SUBMITTER: Guy Sauvageau

PROVIDER: E-GEOD-57561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal.

Fares Iman I Chagraoui Jalila J Gareau Yves Y Gingras Stéphane S Ruel Réjean R Mayotte Nadine N Csaszar Elizabeth E Knapp David J H F DJ Miller Paul P Ngom Mor M Imren Suzan S Roy Denis-Claude DC Watts Kori L KL Kiem Hans-Peter HP Herrington Robert R Iscove Norman N NN Humphries R Keith RK Eaves Connie J CJ Cohen Sandra S Marinier Anne A Zandstra Peter W PW Sauvageau Guy G

Science (New York, N.Y.) 20140901 6203

The small number of hematopoietic stem and progenitor cells in cord blood units limits their widespread use in human transplant protocols. We identified a family of chemically related small molecules that stimulates the expansion ex vivo of human cord blood cells capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. The potent activity of these newly identified compounds, UM171 being the prototype, is independent of suppression of the aryl hydrocarbon rec ...[more]

PMID: 25237102

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