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DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

ABSTRACT: We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites.

ORGANISM(S): Mus musculus

PROVIDER: GSE60075 | GEO | 2015/06/29

SECONDARY ACCESSION(S): PRJNA257422

REPOSITORIES: GEO

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DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites. We used round spermatids as control and analysed the DNA methylation profiles of all the cell lines were by RRBS.

2015-06-29 | E-GEOD-60075 | biostudies-arrayexpress

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Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

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Gene expression analysis and DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:This SuperSeries is composed of the SubSeries listed below.

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CGH analysis of AG-haESCs (androgenetic haploid embryonic stem cells)

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Gene expression analysis and DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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RNA Seq analyzed the transcriptional difference between the ICSI and ICAHCI embryo in blastocyst and placenta

Project description:Our lab first derived mouse androgenetic haploid embryonic stem cells (AG-haESCs) and demonstrated that AG-haESCs can be used as an “artificial spermatids” to generate gene-edited semi-cloned (SC) mice through intracytoplasmic injection (ICAHCI) into mature oocyte, even though the birth efficiency is very low. Further we proved that H19-DMR and IG-DMR were the main barrier to generate viable mice through androgenetic and parthenogenetic haESCs. More importantly, AG-haESCs mediated SC technology combined with CRISPR-Cas9 is a powerful tool to generate gene-modified mouse models and carry out genetic screening at organismal level. However, it is still not clear how the H19-DMR and IG-DMR coordinately regulate SC embryo development. Here, we found that the H19-DMR and IG-DMR regulate the development of SC embryos in spatio-temporal scales. Firstly, we found that the H19-DMR and IG-DMR are not indispensable for the development of preimplantation of SC embryos. Secondly, H19-DMR is essential for the development of SC embryos in mid-gestation and IG-DMR takes effect in late-gestation. Further, the maintenance of paternal H19-DMR methylation status and deletion of paternal H19 transcription unit play a key role in the structures and transport functions of SC embryo placenta. Importantly, AG-haESCs carrying triple deletions, including H19, H19-DMR and IG-DMR, can further improve the efficiency in generation of viable, normal-size, and fertile mice.

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Round Spermatids and Pachytene Spermatocytes

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