Unknown,Transcriptomics,Genomics,Proteomics

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ChIp-on-chip with Rbp3 in C-ternimal domain (CTD) of Rbp1 mutants upon Nacl Stress in yeast


ABSTRACT: The modification of Ser 5 is important for the relocalization of RNAP II upon NaCl stress. The CTD14 strains harbors a plasmid expressing RPB1 with 14 wild-type CTD repeats. The 5A strain carries a plasmid expressing a chimeric RPB1 in which the CTD was composed of 5 repeats of CTD-serine 5 substituted with alanine followed by 7 wild-type-sequenced repeats. Two-color fluorescence arrays reporting on Rpb3 localization abundance in strains (input vs. IP) before and at 20 min after a shock with 0.7M NaCl

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Audrey Gasch 

PROVIDER: E-GEOD-60707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pathway connectivity and signaling coordination in the yeast stress-activated signaling network.

Chasman Deborah D   Ho Yi-Hsuan YH   Berry David B DB   Nemec Corey M CM   MacGilvray Matthew E ME   Hose James J   Merrill Anna E AE   Lee M Violet MV   Will Jessica L JL   Coon Joshua J JJ   Ansari Aseem Z AZ   Craven Mark M   Gasch Audrey P AP  

Molecular systems biology 20141119


Stressed cells coordinate a multi-faceted response spanning many levels of physiology. Yet knowledge of the complete stress-activated regulatory network as well as design principles for signal integration remains incomplete. We developed an experimental and computational approach to integrate available protein interaction data with gene fitness contributions, mutant transcriptome profiles, and phospho-proteome changes in cells responding to salt stress, to infer the salt-responsive signaling net  ...[more]

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