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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome sequencing for cancer stem cell properties in MCF-7 cells with knocked down CD24

ABSTRACT: CD24 is the one of cell surface protein that anchored via glycosyl phosphatidylinositol that links to cell surface and modulates growth and differentiation signal many of cell types. A small population of cells, namely M-bM-^@M-^XCancer Stem Cells (CSCs)M-bM-^@M-^Y, charges highly aggressive and metastatic character of cancer cells and shows conformational change of M-bM-^@M-^Xepithelial to mesenchymal transition (EMT). To understand the role of CD24 in CSC and EMT, CD24 was knocked down using siRNA in the MCF7 cell line (MCF-7 hCD24 KD) and analyzed transcriptome profiles by mRNA-sequencing. Corresponding author: Chul Geun Kim, Department of Life Science, Hanyang University, Seoul 133-791, Korea (e-mail, cgkim@hanyang.ac.kr). mRNA profiles of MCF-7 hCD24 KD cells [M-bM-^@M-^Xmonolayer (m) cultured MCF7 wild type (WT) and mMCF7 CD24KDM-bM-^@M-^Y and M-bM-^@M-^Xsphere-forming (s) MCF7 WT and sMCF7 CD24KDM-bM-^@M-^Y] were generated by deep sequencing using Illumina GAIIx.

ORGANISM(S): Homo sapiens

SUBMITTER: Jung Min Kim

PROVIDER: E-GEOD-61917 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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