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Genome-wide analysis of gene expression in mLTC-1 cells following siRNA-mediated Gata4 inhibition

ABSTRACT: The effects of Gata4 inhibition on global gene expression in mLTC-1 cells was analysed. Using microarray analysis we identified a set of genes that are downregulated or upregulated following siRNA-mediated silencing of Gata4 in the murine Leydig tumor cell line mLTC-1. Among the downregulated genes were enzymes of the androgen biosynthetic pathway. mLTC-1 cells were transfected with non-targeting (NT) siRNA and Gata4 siRNA respectively. 72h post transfection cells were lysed and RNA was extracted. n=3 of each sample group; control samples are samples 3-6 (mLTC-1 cells treated with non-targeting siRNA)

ORGANISM(S): Mus musculus

SUBMITTER: Markku Heikinheimo

PROVIDER: E-GEOD-63320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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GATA4 is a key regulator of steroidogenesis and glycolysis in mouse Leydig cells.

Schrade Anja A Kyrönlahti Antti A Akinrinade Oyediran O Pihlajoki Marjut M Häkkinen Merja M Fischer Simon S Alastalo Tero-Pekka TP Velagapudi Vidya V Toppari Jorma J Wilson David B DB Heikinheimo Markku M

Endocrinology 20150210 5

Transcription factor GATA4 is expressed in somatic cells of the mammalian testis. Gene targeting studies in mice have shown that GATA4 is essential for proper differentiation and function of Sertoli cells. The role of GATA4 in Leydig cell development, however, remains controversial, because targeted mutagenesis experiments in mice have not shown a consistent phenotype, possibly due to context-dependent effects or compensatory responses. We therefore undertook a reductionist approach to study the ...[more]

PMID: 25668067

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Genome-wide analysis of gene expression in TM4 Sertoli cells following siRNA-mediated Gata4 inhibition

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